Modification of host and bacterial proteomes during host-pathogen interactions
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[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Plant cells, such as those of Arabidopsis thaliana, detect the presence of bacteria through cell-surface receptors that recognize microbial-associated molecular patterns (MAMPs). MAMPs are conserved features among microbes and are fundamental to the microbial lifestyle. Plant defense responses aimed at limiting microbial proliferation are elicited when a receptor binds its MAMP ligand. To date, many of the studies on plant-bacteria interactions at the protein level have only examined plant responses, and not bacterial responses. Furthermore, the plant responses were elicited with a single MAMP, instead of the entire bacterium. In the present studies, we used an Arabidopsis cell culture system to examine proteomic changes in both the bacterial pathogen and the plant host cells during the recognition and response of intact host and bacteria. The proteomic changes in the bacteria clearly demonstrated that the bacterial pathogen recognizes host cells and alters its proteome. We also analyzed responses in the host cells during infection with Pto DC3000 (hrcC-). These studies provided a number of new candidates for proteins that may be involved in plant innate immune responses. Taken together, these studies provide new insights into the mutual recognition processes occurring in both the host and pathogen cells that may underlie either resistant or susceptible interactions.
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