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dc.contributor.advisorHill, Michael A.eng
dc.contributor.authorStupica, Aaron J.eng
dc.date.issued2011eng
dc.date.submitted2011 Falleng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on June 8, 2012).eng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.eng
dc.descriptionThesis advisor: Dr. Michael A. Hilleng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionM.S. University of Missouri--Columbia 2011.eng
dc.description"December 2011"eng
dc.description.abstractAdventitial elastin provides structural and mechanical support for arterioles. While elastin quantities are known in arterioles, the structure of elastin fibers and distribution of elastin throughout the vessel was unknown. This project aimed to develop an imaging technique using numerous dyes and 3D confocal imaging to further understanding of arteriolar elastin. Elastin content was stained with Alexa 633 (ex 633nm) and an elastin antibody (ex 488nm). Cell nuclei were stained with Yo-Pro Iodide (ex 488nm). Arterioles from the cremaster muscle of rats were imaged first and showed elastin in the internal and external elastic laminas (IEL and EEL respectively) as well as a vast network of adventitial elastin fibers. In comparison to these vessels, small arteries were isolated and imaged from the cerebral tissue. There was a complete absence of elastin content throughout the vessel, except for the IEL. Finally, small arteries were imaged from the mesentery. The elastin content in the mesenteric vessels was similar to that from the cremaster arteriole. There was an expansive network of adventitial elastin as well as significant elastin content in the IEL and EEL. The difference in adventitial elastin may be due to the mechanical environment that each vessel was isolated from. Using the imaging protocol developed, vessels can be imaged while focusing on age or disease to further understanding about the physiological changes that occur in these states.eng
dc.description.sponsorshipAdventitial elastin provides structural and mechanical support for arterioles. While elastin quantities are known in arterioles, the structure of elastin fibers and distribution of elastin throughout the vessel was unknown. This project aimed to develop an imaging technique using numerous dyes and 3D confocal imaging to further understanding of arteriolar elastin. Elastin content was stained with Alexa 633 (ex 633nm) and an elastin antibody (ex 488nm). Cell nuclei were stained with Yo-Pro Iodide (ex 488nm). Arterioles from the cremaster muscle of rats were imaged first and showed elastin in the internal and external elastic laminas (IEL and EEL respectively) as well as a vast network of adventitial elastin fibers. In comparison to these vessels, small arteries were isolated and imaged from the cerebral tissue. There was a complete absence of elastin content throughout the vessel, except for the IEL. Finally, small arteries were imaged from the mesentery. The elastin content in the mesenteric vessels was similar to that from the cremaster arteriole. There was an expansive network of adventitial elastin as well as significant elastin content in the IEL and EEL. The difference in adventitial elastin may be due to the mechanical environment that each vessel was isolated from. Using the imaging protocol developed, vessels can be imaged while focusing on age or disease to further understanding about the physiological changes that occur in these states.eng
dc.format.extentxvi, 96 pageseng
dc.identifier.urihttp://hdl.handle.net/10355/14573
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.subjectthree dimensional confocal imagingeng
dc.subjectmicrovascular mechanicseng
dc.subjectarterioleeng
dc.subjectelastin fibereng
dc.subjectimaging protocoleng
dc.titleRegional heterogeneity of adventitial elastin in the arteriolar wall reflects differences in the mechanical environmenteng
dc.typeThesiseng
thesis.degree.disciplineBiological engineering (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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