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dc.contributor.advisorKirk, Mark D.eng
dc.contributor.authorDunham, Cheryl Lynneng
dc.date.issued2011eng
dc.date.submitted2011 Falleng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on June 11, 2012).eng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.eng
dc.descriptionThesis advisor: Dr. Mark D. Kirkeng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionM.A. University of Missouri-Columbia 2011.eng
dc.description"December 2011"eng
dc.description.abstractEmbryonic stem (ES) cells can differentiate into many specialized cell types, including neural cells. Numerous induction protocols have been developed to direct their differentiation. Our lab created an in vitro neural stem cell (NSC) niche through the induction of 129 derived B5 ES cells. Subsequent attempts using the established in vitro NSC niche protocol on the B6 ES cell line (derived from the C57BL/6 mouse strain) were unsuccessful. I proceeded to characterize the B6 ES cell line. B6 embryonic stem cells grew significantly slower than B5 ES cells under similar conditions. After application of the 4-/4+ retinoic acid (RA) neural induction protocol, B6 embryoid bodies (EBs) displayed a neural rosette-like morphology. Immunohistochemical labeling of the Day 8 EBs revealed a labeling pattern that suggests the EBs may be recapitulating the inside-out formation of cortical development in mammals. When dissociated Day 8 B6 EB cells were transplanted into the left striatum of syngeneic C57BL/6 mice, teratomas formed. The slow growth rate of B6 cells may have contributed to their incomplete neuralization, and to achieve a more complete neural induction, a modified RA induction protocol should be considered for use in future studies.eng
dc.format.extentv, 59 pageseng
dc.identifier.urihttp://hdl.handle.net/10355/14593
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.source.originalSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subjectembyonic stem celleng
dc.subjectneural stem celleng
dc.subjectneural rosetteeng
dc.subjectcorticogenesiseng
dc.subjectteratomaeng
dc.titleTeratoma formation by neuralized C57BL/6J-EGFP mouse embyonic stem cells after syngeneic transplantation into the midbraineng
dc.typeThesiseng
thesis.degree.disciplineBiological sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.A.eng


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