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dc.contributor.advisorDavis, Michael J., 1953 Nov.-eng
dc.contributor.authorSchwindt, Amy E.eng
dc.date.issued2011eng
dc.date.submitted2011 Falleng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on June 11, 2012).eng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionThesis advisor: Dr. Michael J. Daviseng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionM.S. University of Missouri-Columbia 2011.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- physiology (Medicine).eng
dc.description"December 2011"eng
dc.description.abstractDuring vascular morphogenic events, mural cells are recruited to developing endothelial tubes to aid in stabilization and maturation of the new vessels. There are two known types of mural cells, pericytes and vascular smooth muscle cells (VSMCs). Their different locations within the vasculature (capillaries versus larger vessels, respectively) suggest that different signals may be responsible for recruiting each cell type. Platelet-­‐derived growth factor (PDGF) is released from endothelial cells (ECs) during morphogenic events and has been shown to influence mural cell functions. In this study, I investigated the ability of PDGF isoforms to regulate pericyte and VSMC invasiveness and subsequent effects on EC monolayer stability and tube sprouting. Using novel cell-­‐invasion systems developed during this research, I show that PDGF isoforms that bind to PDGFRβ induce pericyte, but not VSMC invasion. Coculture studies revealed that the invasive behavior of pericytes is critical to both EC stability and sprouting. When ECs were seeded as a monolayer on top of 3D collagen gels containing pericytes, monolayer stability was enhanced compared to EC monolayers seeded alone or with VSMCs. Coculture studies also revealed that EC sprouting was sustained when pericytes were present compared to EC only cultures or EC/VSMC cocultures. Results from this work define functional differences between pericytes and VSMCs during vascular morphogenesis.eng
dc.format.extentviii, 57 pageseng
dc.identifier.urihttp://hdl.handle.net/10355/14599
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartof2011 Freely available theses (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2011 Theseseng
dc.subjectmural cellseng
dc.subjectvascular morphogenesiseng
dc.subjectpericyteseng
dc.subjectplatelet-­‐derived growth factoreng
dc.titleFunctional differences between pericytes and vascular smooth muscle cells during vascular morphogenesiseng
dc.typeThesiseng
thesis.degree.disciplinePhysiology (Medicine) (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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