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dc.contributor.authorMeyer, Mattheweng
dc.contributor.corporatenameUniversity of Missouri-Columbia. Office of Undergraduate Researcheng
dc.contributor.meetingnameUndergraduate Research and Creative Achievements Forum (2006 : University of Missouri--Columbia)eng
dc.date2006eng
dc.date.issued2006eng
dc.descriptionAbstract only availableeng
dc.description.abstractFall armyworm (FAW) and southwestern corn borer (SWCB) are insect pests of maize which cause large amounts of feeding damage annually in the United States. Previous proteomic analysis of resistant and susceptible lines of maize showed proteins involved with the photosynthetic pathway were differentially expressed in the resistant line. The high chlorophyll fluorescent (hcf) mutants contain defects in the photsystem I or II pathways. These defects lead to disrupted electron transport within the photosynthetic pathway. Preference tests comparing several hcf mutants to wild-type siblings determined genes involved in photosynthesis had an effect on insect feeding. Antibiosis tests were performed using mutant and wild-type leaf tissue to determine their effect on larval weight gain. Leaf feeding damage was assessed using the AlphaEase software from digital images. Individual larval weights were determined in milligrams. hcf7-N1029D significantly decreased larval feeding damage for both FAW and SWCB. hcf*-88-3005-3, hcf11-N1250A, and hcf48-N1282C significantly increased larval feeding damage for both Lepidoptera. The combined data from both the antibiosis and preference tests reveal that photosystem II genes have a significant effect on larval feeding which is unrelated to leaf color. Further, these genes are contained within the stromal thylakoid where fatty acid precursors are produced for epicuticular wax synthesis. Previous data has determined epicuticular waxes exhibit resistance to Lepidopteran insects. Electron microscopy was performed on several hcf mutants to determine their effects on the structure of epicuticular waxes. Comparisons between mutant and wild-type tissue show substantial differences in wax distribution and accumulation.eng
dc.description.sponsorshipMU Monsanto Undergraduate Research Fellowshipeng
dc.identifier.urihttp://hdl.handle.net/10355/1478eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri - Columbia Office of Undergraduate Researcheng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forumeng
dc.source.urihttp://undergradresearch.missouri.edu/forums-conferences/abstracts/abstract-detail.php?abstractid=eng
dc.subjectmaizeeng
dc.subjectinsect infestationeng
dc.subjecthigh chlorophyll fluorescent (hcf) mutantseng
dc.subjectfeeding damageeng
dc.titlePhotosytem II genes display a potential mechanism of Lepidopteran resistance [abstract]eng
dc.typePresentationeng


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