Quantification of Low-level GM Seed Presence in Canadian Commercial Flax Stocks

MOspace/Manakin Repository

Breadcrumbs Navigation

Quantification of Low-level GM Seed Presence in Canadian Commercial Flax Stocks

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/14914

[+] show full item record


Title: Quantification of Low-level GM Seed Presence in Canadian Commercial Flax Stocks
Author: Booker, Helen; Lamb, Eric
Keywords: CDC Triffid
flax (Linum usitatissimum)
GMO
seed purity analysis
seed testing
statistical methods
transgenic seeds
Date: 2012
Publisher: AgBioForum
Citation: AgBioForum, 15(1), 31-35.
Abstract: Detection and quantification of the prevalence of genetically modified (GM) organism contamination in seed exports is a critical element of regulatory compliance. While the procedures to reliably detect high levels of GM contamination are well understood, no comparable statistical approaches are available for the quantification of levels of GM prevalence below the established detection rate of standard tests. Presented is a simple statistical approach based on simulation modeling for the quantification of low levels of GM contamination. The approach can be modified to match any sampling regime and can account for rates of false positive and negative assay results. The application of this method is demonstrated using the low level of contamination in Canadian commercial flax stocks by the GM flax variety “Triffid.” We show that rates of GM contamination in commercial flax stocks ranged between 1 GM seed per million and 1 seed per hundred thousand. A simulation model was used to determine whether the observed rates of positive tests are within the range expected from false positive rates of the test. We showed that for the majority of categories of grain or seed, the very low level of GM prevalence still remains outside that which is to be expected based on false positives returned or by chance alone. These results indicate a pervasive low-level presence of GM construct in the Canadian commercial flax system.
URI: http://hdl.handle.net/10355/14914
ISSN: 1522-936X

This item appears in the following Collection(s)

[+] show full item record