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dc.contributor.advisorThelen, Jay J.eng
dc.contributor.authorStevenson, Severin E.eng
dc.date.issued2012eng
dc.date.submitted2012 Springeng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on September 7, 2012).eng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionDissertation advisor: Dr. Jay J. Theleneng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionVita.eng
dc.descriptionPh. D. University of Missouri-Columbia 2012.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biochemistry.eng
dc.description"May, 2012"eng
dc.description.abstract[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Incidences of food allergies have doubled in the past two decades, becoming a worldwide public health concern. Together, tree nuts and peanuts cause approximately 80% of anaphylactic reactions and account for over 50% of child fatalities related to food allergies. For this reason, accurate and sensitive methods are necessary to identify and quantify seed allergens as a means to provide consumer information on seed and seed-derived food-products. New technologies (e.g. proteomics and mass spectrometry) can provide the means to compare allergen concentrations among seed varieties to evaluate the contribution of both genetic and environmental factors. The work presented, describes the development of quantitative mass spectrometry techniques and their application to the study of seed composition dynamics. Firstly, the effects of RNAi-mediated reduction of major seed allergens (Ara h 2 and 6) on seed protein composition were studied using spectral counting and the Absolute QUAntitation strategy (AQUA). Using these methods, the available proteome was surveyed for major alterations in both on-target (Ara h 2 and 6) and potential off-target proteins. Several off-target alterations were discovered including an increase in another known allergen. Secondly, the AQUA strategy was further applied to study the effects of environment and genotype on allergens and anti-nutritional proteins in soybean. In this study we analyzed the levels of known allergens and anti-nutritional proteins in commercially available soybean lines grown in six locations spanning three climate zones in North America. Robust statistical analysis shows that the effects of environment far outweigh those of genotype.--From public.pdfeng
dc.format.extentix,155 pageseng
dc.identifier.oclc872569334eng
dc.identifier.urihttps://hdl.handle.net/10355/15194
dc.identifier.urihttps://doi.org/10.32469/10355/15194eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertations.eng
dc.rightsAccess is limited to the campus of the University of Missouri--Columbia.eng
dc.source.originalSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subjectgenetic modificationeng
dc.subjectfood allergyeng
dc.subjectallergen concentrationseng
dc.subjectenvironmental factoreng
dc.titleMass spectrometry-based seed allergen quantification and applications in seed quality assessmenteng
dc.typeThesiseng
thesis.degree.disciplineBiochemistry (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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