Meiotic silencing by unpaired DNA: elucidation of a new gene silencing pathway

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Meiotic silencing by unpaired DNA: elucidation of a new gene silencing pathway

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/15761

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dc.contributor.advisor Shiu, Patrick en_US
dc.contributor.author Alexander, William
dc.contributor.other University of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2011 Dissertations en_US
dc.date.accessioned 2012-10-18T14:13:25Z
dc.date.available 2012-10-18T14:13:25Z
dc.date.issued 2011
dc.date.submitted 2011 Spring en_US
dc.identifier.other AlexanderW-050911-D5244
dc.identifier.uri http://hdl.handle.net/10355/15761
dc.description Title from PDF of title page (University of Missouri--Columbia, viewed on October 18, 2012). en_US
dc.description The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. en_US
dc.description Dissertation advisor: Dr. Patrick K. T. Shiu en_US
dc.description Includes bibliographical references. en_US
dc.description Vita. en_US
dc.description Ph. D. University of Missouri-Columbia 2012. en_US
dc.description Dissertations, Academic -- University of Missouri--Columbia -- Biological sciences. en_US
dc.description "May 2011" en_US
dc.description.abstract Meiotic silencing by unpaired DNA is an RNAi-mediated pathway that functions to silence unpaired genes during the sexual phase of Neurospora crassa. The presence of a gene insertion or deletion causes an unpairing event during the homolog pairing stage. My dissertation's aim was to further characterize this new RNAi pathway. First, I was involved in adapting the in vivo protein interaction technique Bimolecular Fluorescence Complementation (BiFC) to N. crassa to investigate SAD-1/SAD-2 interaction. SAD-1 and SAD-2 interact in the perinuclear region, suggesting that SAD-2 functions to bring SAD-1 to its proper location. Next, I was involved in identifying components of quelling that also function in meiotic silencing. dcl-1 and qip were found to be important for sexual development and meiotic silencing. DCL-1 and QIP also localize in the perinuclear region, implicating these genes in meiotic silencing. Finally, I tested the mating type locus for immunity to meiotic silencing. Unpaired reporter gene insertions resulted in ascospore populations exhibiting partial protection from silencing. When a reporter gene was enclosed with paired mat locus flanks at an ectopic locus, partial protection was again observed. en_US
dc.format.extent xiv, 96 pages en_US
dc.language.iso en_US en_US
dc.publisher University of Missouri--Columbia en_US
dc.relation.ispartof 2011 Freely available dissertations (MU) en_US
dc.subject RNA interference en_US
dc.subject neurospora en_US
dc.subject fungal genetics en_US
dc.subject meiotic silencing en_US
dc.subject sexual development en_US
dc.title Meiotic silencing by unpaired DNA: elucidation of a new gene silencing pathway en_US
dc.type Thesis en_US
thesis.degree.discipline Biological sciences en_US
thesis.degree.grantor University of Missouri--Columbia en_US
thesis.degree.name Ph. D. en_US
thesis.degree.level Doctoral en_US


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