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dc.contributor.advisorShiu, Patricken_US
dc.contributor.authorAlexander, William
dc.contributor.otherUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2011 Dissertationsen_US
dc.date.issued2011
dc.date.submitted2011 Springen_US
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on October 18, 2012).en_US
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.en_US
dc.descriptionDissertation advisor: Dr. Patrick K. T. Shiuen_US
dc.descriptionIncludes bibliographical references.en_US
dc.descriptionVita.en_US
dc.descriptionPh. D. University of Missouri-Columbia 2012.en_US
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biological sciences.en_US
dc.description"May 2011"en_US
dc.description.abstractMeiotic silencing by unpaired DNA is an RNAi-mediated pathway that functions to silence unpaired genes during the sexual phase of Neurospora crassa. The presence of a gene insertion or deletion causes an unpairing event during the homolog pairing stage. My dissertation's aim was to further characterize this new RNAi pathway. First, I was involved in adapting the in vivo protein interaction technique Bimolecular Fluorescence Complementation (BiFC) to N. crassa to investigate SAD-1/SAD-2 interaction. SAD-1 and SAD-2 interact in the perinuclear region, suggesting that SAD-2 functions to bring SAD-1 to its proper location. Next, I was involved in identifying components of quelling that also function in meiotic silencing. dcl-1 and qip were found to be important for sexual development and meiotic silencing. DCL-1 and QIP also localize in the perinuclear region, implicating these genes in meiotic silencing. Finally, I tested the mating type locus for immunity to meiotic silencing. Unpaired reporter gene insertions resulted in ascospore populations exhibiting partial protection from silencing. When a reporter gene was enclosed with paired mat locus flanks at an ectopic locus, partial protection was again observed.en_US
dc.format.extentxiv, 96 pagesen_US
dc.identifier.otherAlexanderW-050911-D5244
dc.identifier.urihttp://hdl.handle.net/10355/15761
dc.publisherUniversity of Missouri--Columbiaen_US
dc.relation.ispartof2011 Freely available dissertations (MU)en_US
dc.source.originalSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subjectRNA interferenceen_US
dc.subjectneurosporaen_US
dc.subjectfungal geneticsen_US
dc.subjectmeiotic silencingen_US
dc.subjectsexual developmenten_US
dc.titleMeiotic silencing by unpaired DNA: elucidation of a new gene silencing pathwayen_US
dc.typeThesisen_US
thesis.degree.disciplineBiological sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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