Shared more. Cited more. Safe forever.
    • advanced search
    • submit works
    • about
    • help
    • contact us
    • login
    View Item 
    •   MOspace Home
    • University of Missouri-Columbia
    • Graduate School - MU Theses and Dissertations (MU)
    • Theses and Dissertations (MU)
    • Dissertations (MU)
    • 2011 Dissertations (MU)
    • 2011 MU dissertations - Freely available online
    • View Item
    •   MOspace Home
    • University of Missouri-Columbia
    • Graduate School - MU Theses and Dissertations (MU)
    • Theses and Dissertations (MU)
    • Dissertations (MU)
    • 2011 Dissertations (MU)
    • 2011 MU dissertations - Freely available online
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    advanced searchsubmit worksabouthelpcontact us

    Browse

    All of MOspaceCommunities & CollectionsDate IssuedAuthor/ContributorTitleSubjectIdentifierThesis DepartmentThesis AdvisorThesis SemesterThis CollectionDate IssuedAuthor/ContributorTitleSubjectIdentifierThesis DepartmentThesis AdvisorThesis Semester

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular AuthorsStatistics by Referrer

    Evaluation of in vitro and in vivo equine microbial population shifts in response to colic conditions

    Venable, Erin
    View/Open
    [PDF] public.pdf (6.750Kb)
    [PDF] research.pdf (1019.Kb)
    [PDF] short.pdf (6.750Kb)
    Date
    2011
    Format
    Thesis
    Metadata
    [+] Show full item record
    Abstract
    We designed two continuous culture experiments to test the effects of starch dosage and starvation upon equine fecal microflora. Diets were designed containing fructooligosaccharides and mannanoligosaccharides in order to investigate treatment effects on VFA and NH3 concentration, and pH following starvation or starch overload. This research indicated that total volatile fatty acid (VFA) concentration may be affected by treatment following starch overload. Additionally, branched chain VFA, and butyric acid concentrations were different across treatment in both experiments. This research indicated that following an abrupt change in the starch content of the diet, or a starvation period, those effects may be ameliorated by diet. In our third experiment, we performed pyrosequencing in order to phylogenetically characterize the shifts in the microbial populations observed in the feces of eight horses collected from both the colic (large intestinal-nonsurgical) and healthy states. Healthy samples were collected by the attending/referring veterinarian 60 days ([plus or minus] 30) post discharge. DNA was extracted from the fecal samples and the V1, V2, and V3 region of the 16S rDNA was amplified via emulsion PCR. When ubiquitous microbes were ranked colic > healthy, four were significant. All sick horses had greater reads of Clostridium phytofermentans, an uncultured Clostridiales bacterium, and Bacteroidetes as well as an uncultured bacteroidetes bacterium. These data suggest there is a need for broad bacterial diversity in the healthy equine gut.
    URI
    https://doi.org/10.32469/10355/15820
    https://hdl.handle.net/10355/15820
    Degree
    Ph. D.
    Thesis Department
    Animal sciences (MU)
    Rights
    OpenAccess.
    This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
    Collections
    • Animal Sciences electronic theses and dissertations (MU)
    • 2011 MU dissertations - Freely available online

    Send Feedback
    hosted by University of Missouri Library Systems
     

     


    Send Feedback
    hosted by University of Missouri Library Systems