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dc.contributor.advisorAlmasri, Mahmoudeng
dc.contributor.authorCheng, Qieng
dc.date.issued2011eng
dc.date.submitted2011 Springeng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on October 24, 2012).eng
dc.descriptionThe entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.eng
dc.descriptionDissertation advisor: Dr. Mahmoud Almasrieng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionVita.eng
dc.descriptionPh. D. University of Missouri--Columbia 2011.eng
dc.description"May 2011"eng
dc.description.abstractThis thesis describes the design, modeling, fabrication and characterization of a micromachined array of high density 3-dimensional microposts (100 x 100) made of flexible material (silicone elastomers) for use to measure quantitatively the cellular traction force generated by vascular smooth muscle cell (VSMC) with high sensitivity and accuracy. The micropost arrays were then fabricated with diameters ranged from 3 to 10 μm, with edge to edge spacing of 5 and 7 [mu]m, and with a height to diameter aspect radio up to 13 using microfabrication techniques and replica molding. The mechanical properties of the Polydimethylsiloxane (PDMS) microposts with various geometries used in the cell culture experiment were determined experimentally including detailed measurements of Young's modulus (E) and the corresponding spring constant. We have found that microposts with different sizes and geometries have different Young's. Vascular smooth muscle cells were cultured on top of the micropost arrays and incubated for 2 days before an image acquisition experiment. The micropost arrays with different geometries were used to study VSMCs. We have found that the traction force exerted by VSM cell increases as the stiffness of the micropost increases. It demonstrates that VSM cell tends to adjust its traction force to adapt to its physical environment. VSMCs with integrin-linked kinase enzyme (ILKE), referred to as CK4 cell, and without ILK module, referred as ILK cell, were also studied using PDMS micropost array. This study indicates that the function of ILK molecule is involved to the VSMC contraction and the control its traction force. Finally, high temporal resolution analysis of CK4 cells was performed on PDMS micropost array.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.format.extentxviii, 129 pageseng
dc.identifier.oclc872561679eng
dc.identifier.urihttps://doi.org/10.32469/10355/15833eng
dc.identifier.urihttps://hdl.handle.net/10355/15833
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertations.eng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.subjectcellular traction forceeng
dc.subjectmicropost arrayeng
dc.subjectpolydimethylsiloxaneeng
dc.subjectYoung's moduluseng
dc.titleMicromachined PDMS elastic post arrays for studying vascular smooth muscle cellseng
dc.typeThesiseng
thesis.degree.disciplineElectrical and computer engineering (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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