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dc.contributor.authorCooper, Charles B.eng
dc.contributor.authorCharlson, Dirk V.eng
dc.contributor.authorMaurer, Alberto, 1970-eng
dc.contributor.authorRogers, Elizabeth E.eng
dc.contributor.corporatenameUniversity of Missouri-Columbia. Office of Undergraduate Researcheng
dc.contributor.meetingnameUndergraduate Research and Creative Achievements Forum (2007 : University of Missouri--Columbia)eng
dc.date2007eng
dc.date.issued2007eng
dc.descriptionAbstract only availableeng
dc.description.abstractIron is essential for virtually all life, but little is known about the way plants sense, take up, and maintain iron. In an effort to learn more about how plants use this vital nutrient, two projects using the model plant Arabidopsis thaliana were undertaken. The first was a novel genetic screen using transgenic plants harboring the reporter Green Fluorescent Protein (GFP) driven by the iron-regulated AtFer1 promoter. One mutant, OAF 102, was identified for its constitutively high GFP fluorescence relative to unmutagenized transgenic controls. OAF 102 has constitutively elevated GFP mRNA but retains iron-dependent expression of AtFer1; all other iron-related traits tested were similar in OAF 102 and the transgenic parent. Sequencing of the transgenic promoter from OAF 102 did not reveal any mutations in the TATA box, G Box, or iron-dependent regulatory sequence (IDRS). The decoupled regulation of these genes may indicate an epigenetic mutation or a mutation elsewhere in the genome. The other project is an investigation of mutant lines singly and doubly mutant for T-DNA insertions in four bHLH transcription factors found to be upregulated during iron deficiency. Neither single nor double mutant lines showed alterations in ferric-chelate reductase activity. This may reflect a role in iron homeostasis that is not detectable using this tool or a degree of functional redundancy. Further investigation of these T-DNA lines and of OAF 102 is ongoing.eng
dc.description.sponsorshipMU Monsanto Undergraduate Research Fellowshipeng
dc.identifier.urihttp://hdl.handle.net/10355/1584eng
dc.languageen_USeng
dc.publisherUniversity of Missouri--Columbia. Office of Undergraduate Researcheng
dc.relation.ispartof2007 Undergraduate Research and Creative Achievements Forum (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forumeng
dc.source.urihttp://undergradresearch.missouri.edu/forums-conferences/abstracts/abstract-detail.php?abstractid=eng
dc.subjectGreen Fluorescent Protein (GFP)eng
dc.subjectiron-related traitseng
dc.subjectiron-dependent regulatory sequenceeng
dc.subjectferric-chelate reductase activityeng
dc.titleInvestigations of iron-regulated genes in Arabidopsis thaliana [abstract]eng
dc.typePresentationeng


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