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dc.contributor.authorCrawford, Michaeleng
dc.contributor.authorGordon, Noah M., 1968-eng
dc.contributor.authorTegtmeyer, Chrstophereng
dc.contributor.authorGerhardt, H. Carleng
dc.contributor.corporatenameUniversity of Missouri-Columbia. Office of Undergraduate Researcheng
dc.contributor.meetingnameSummer Undergraduate Research and Creative Achievements Forum (2004 : University of Missouri--Columbia)eng
dc.date2004eng
dc.date.issued2004eng
dc.descriptionAbstract only availableeng
dc.description.abstractEthyl 3-aminobenzoate (MS-222) is commonly used to anesthetize fish and amphibians, but few studies have investigated its effects on frogs. We experimented with the effects of combining the use of MS-222 with the extraction of blood via heart puncture. We developed a protocol for the use of MS-222 on male gray treefrogs (Hyla versicolor). We exposed the frogs to MS-222 for different time intervals, then extracted blood while they were unconscious. Once blood had been taken, the frogs were closely monitored to see how long it took them to regain consciousness, if in fact they did so. Exposures of 7 min and above to MS-222 resulted in high mortality, but exposures of 4 min or less were sufficient for blood extraction with little or no mortality or behavioral indications of pain or stress. This experiment could serve as a template for other anurans closely related to Gray Treefrogs (H. versicolor).eng
dc.description.sponsorshipNSF-REU Biology & Biochemistryeng
dc.identifier.urihttp://hdl.handle.net/10355/1588eng
dc.languageen_USeng
dc.publisherUniversity of Missouri--Columbia. Office of Undergraduate Researcheng
dc.relation.ispartof2004 Summer Undergraduate Research and Creative Achievements Forum (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forumeng
dc.source.urihttp://undergradresearch.missouri.edu/forums-conferences/abstracts/abstract-detail.php?abstractid=eng
dc.subjectgray treefrogs (hyla versicolor)eng
dc.subjectethyl 3-aminobenzoate (MS-222)eng
dc.subjectanesthetizationeng
dc.titleDetermining the feasibility of anesthetization with a MS-222 solution in combination with cardiocentesis in male gray treefrogs (hyla versicolor)eng
dc.typePresentationeng


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