Shared more. Cited more. Safe forever.
    • advanced search
    • submit works
    • about
    • help
    • contact us
    • login
    View Item 
    •   MOspace Home
    • University of Missouri-Kansas City
    • School of Graduate Studies (UMKC)
    • Theses and Dissertations (UMKC)
    • Theses (UMKC)
    • 2012 Theses (UMKC)
    • 2012 UMKC Theses - Freely Available Online
    • View Item
    •   MOspace Home
    • University of Missouri-Kansas City
    • School of Graduate Studies (UMKC)
    • Theses and Dissertations (UMKC)
    • Theses (UMKC)
    • 2012 Theses (UMKC)
    • 2012 UMKC Theses - Freely Available Online
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    advanced searchsubmit worksabouthelpcontact us

    Browse

    All of MOspaceCommunities & CollectionsDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis SemesterThis CollectionDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis Semester

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular AuthorsStatistics by Referrer

    A genetic linkage map of the fungus phycomyces blakesleeanus for gene identification by map-based cloning

    Chaudhary, Suman
    View/Open
    [PDF] A genetic linkage map of the fungus phycomyces blakesleeanus for gene identification by map-based cloning (3.895Mb)
    Date
    2012
    Format
    Thesis
    Metadata
    [+] Show full item record
    Abstract
    The Mucoromycotina is one of the basal lineages in the fungi, all of which are poorly understood. Mucormycosis is a life threatening infection caused by fungi of the order Mucorales, the most common species of which is Rhizopus oryzae. Certain genetic traits in fungal genomes are thought to influence their ability to cause disease. This fungus is closely related to Phycomyces blakesleeanus, for certain common properties like taxonomic classification and including response to environmental signals. In view of these common properties, Phycomyces presents itself as an important model to study pathogenic determinants of Rhizopus infection. P. blakesleeanus is a zygomycete fungus classified in the subphylum Mucoromycotina, studied because of its environmental sensing abilities and responses, and its ability to synthesize the pigment beta-carotene. Light is an environmental signal that modulates many aspects of fungal biology including the ability to cause disease. There are eight unknown light-sensing mad genes in Phycomyces. The inability to transform DNA into Phycomyces has blocked the identification of genes in this fungus. In this research, a genetic map based on 100 molecular markers, assigned to 121 progeny, was generated in a cross between two wild type strains NRRL1555 and UBC21. The map comprised 1037.8 cM spread over 11 linkage groups. The map was then used as the starting point for the choice of markers for the map-based identification of madC, by crossing madC mutants to strain UBC21. madC was identified as a new gene required for fungal responses to their environment. The madC gene encodes a Ras GTPase activating protein. These findings indicate that the Ras signal transduction pathway plays role in light sensing. Because both light sensing and Ras signaling are required for virulence in other fungal species, a future direction would be to test if mutation of the madC gene has an effect on disease caused by pathogenic members of the Mucoromycotina. By identifying the mad light-sensing genes of Phycomyces, this research can be potentially translated into therapeutics by drug discovery of those genes with roles in both light-sensing and pathogenicity.
    Table of Contents
    Introduction -- Materials and methods -- Results -- Discussion -- Appendix
    URI
    http://hdl.handle.net/10355/16249
    Degree
    M.S.
    Thesis Department
    Bioinformatics (UMKC)
     
    Cell Biology and Biophysics (UMKC)
     
    Collections
    • Cell Biology and Biophysics Electronic Theses and Dissertations (UMKC)
    • 2012 UMKC Theses - Freely Available Online
    • Biomedical and Health Informatics Electronic Theses (UMKC)

    If you encounter harmful or offensive content or language on this site please email us at harmfulcontent@umkc.edu. To learn more read our Harmful Content in Library and Archives Collections Policy.

    Send Feedback
    hosted by University of Missouri Library Systems
     

     


    If you encounter harmful or offensive content or language on this site please email us at harmfulcontent@umkc.edu. To learn more read our Harmful Content in Library and Archives Collections Policy.

    Send Feedback
    hosted by University of Missouri Library Systems