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dc.contributor.authorGrow, Edwardeng
dc.contributor.authorGarcia, Sherry Flinteng
dc.contributor.authorCone, Karen Camille, 1952-eng
dc.contributor.corporatenameUniversity of Missouri-Columbia. Office of Undergraduate Researcheng
dc.contributor.meetingnameUndergraduate Research and Creative Achievements Forum (2008 : University of Missouri--Columbia)eng
dc.date2008eng
dc.date.issued2008eng
dc.descriptionAbstract only availableeng
dc.description.abstractPl1-Blotched is an epigenetically regulated allele (an epiallele) of the purple plant1 gene, which activates synthesis of purple anthocyanin pigments. We identified a modifier called Suppressor of plant blotching 1 (Spb1), which leads to increased pigmentation in Pl1-Blotched plants. This phenotype is correlated with higher levels of Pl1-Blotched mRNA and an altered pattern of DNA methylation on the Pl1-Blotched gene. These features suggest that the effect of Spb1 may be to epigenetically alter the organization of Pl1-Blotched chromatin. An F2 population generated from a cross between two Pl-Blotched plants (Pl-Blotched; Spb X Pl-Blotched, no Spb) showed a continuous distribution and transgressive segregation suggesting a polygenic trait. Individuals were genotyped at 104 SSR loci and anthocyanin content was quantified. Mapmaker and QTL cartographer software analysis of 211-individual and 279-individual F2 populations both identified QTL on chromosomes 5, 6, 7 contributed by Spb and non-Spb parents. Epistacy and ANOVA analysis of a ~1400 F2 population indicates that epistatic interactions are extremely important for high anthocyanin levels. The QTL intervals identified in both studies contain a number of candidate genes that might influence pigmentation of Pl1-Blotched. One of these genes—intensifier 1--was previously shown to be capable of increasing anthocyanin accumulation, although the mechanism of action is uncertain. Other candidates in the QTL intervals are several chromatin genes with likely, but as yet uncharacterized, roles in epigenetic regulation. The Chromosome 6 QTL spans a region that contains the pl1 locus, however, no sequence polymorphism between the two parental Pl-Blotched alleles has yet been identified. This might suggest that the chromosome 6 QTL is due to chromatin inherited from Spb, rather than DNA polymorphism at pl1.eng
dc.description.sponsorshipNational Science Foundationeng
dc.identifier.urihttp://hdl.handle.net/10355/1906eng
dc.languageen_USeng
dc.publisherUniversity of Missouri--Columbia. Office of Undergraduate Researcheng
dc.relation.ispartof2008 Undergraduate Research and Creative Achievements Forum (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forumeng
dc.subjectepigenetically regulated alleleeng
dc.subjectpolygenic traiteng
dc.subjectpurple anthocyanin pigmenteng
dc.titleQTL mapping of suppressor of plant blotching1, modifiers of an epiallele [abstract]eng
dc.typePresentationeng


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