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dc.contributor.authorMayfield, Dustineng
dc.contributor.authorGrow, Edwardeng
dc.contributor.authorCone, Karen Camille, 1952-eng
dc.contributor.corporatenameUniversity of Missouri-Columbia. Office of Undergraduate Researcheng
dc.contributor.meetingnameUndergraduate Research and Creative Achievements Forum (2008 : University of Missouri--Columbia)eng
dc.date2008eng
dc.date.issued2008eng
dc.descriptionAbstract only availableeng
dc.description.abstractEukaryotes can regulate gene expression by epigenetic mechanisms at the chromatin level. One epigenetically regulated gene in maize is Pl1-Blotched, an allele of purple plant1, which activates anthocyanin pigment production. Normally, Pl1-Blotched leads to a variegated pattern of pigmentation, but its pigment level can be increased by a modifier called Suppressor of plant blotching (Spb). At the molecular level, Spb alters the chromatin packing of Pl1-Blotched, leading to less tightly packed chromatin and higher gene expression. The genetic identity of Spb is not yet known, although preliminary results indicate the Spb-enhanced pigment phenotype is a quantitative trait. As a step toward isolating the genes for Spb, a mapping population of Recombinant Inbred Lines (RILs) was generated by inbreeding the progeny of an F2 derived from a cross of Pl1-Blotched with Spb to a less pigmented Pl1-Blotched stock. Genotypes were determined for 188 RILs using microsatellite or simple sequence repeat (SSR) markers. RILs were evaluated for residual heterozygosity, breakpoint locations, and heterozygous chromosomal positions. A map was created and will be used as the foundation for mapping Spb and for estimating the heritability of the Spb phenotype.eng
dc.description.sponsorshipNSF-REU Program in Biological Sciences & Biochemistryeng
dc.identifier.urihttp://hdl.handle.net/10355/1960eng
dc.publisherUniversity of Missouri--Columbia. Office of Undergraduate Researcheng
dc.relation.ispartof2008 Summer Undergraduate Research and Creative Achievements Forum (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forumeng
dc.subjectepigenetic mechanismseng
dc.subjectquantitative trait locus mappingeng
dc.titleCharacterization of a recombinant inbred line population for quantitative trait locus mappingeng
dc.typePresentationeng


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