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dc.contributor.authorNiebruegge, Bridget A.eng
dc.contributor.authorBeeman, Josepheng
dc.contributor.authorGhormley, Alison L.eng
dc.contributor.authorMyears, Hillary E.eng
dc.contributor.authorNagel, Susan C.eng
dc.contributor.corporatenameUniversity of Missouri-Columbia. Office of Undergraduate Researcheng
dc.contributor.meetingnameUndergraduate Research and Creative Achievements Forum (2008 : University of Missouri--Columbia)eng
dc.date2008eng
dc.date.issued2008eng
dc.descriptionAbstract only availableeng
dc.description.abstractChemicals found in the environment have been found to behave like the body's natural estrogen, estradiol. These exogenous estrogen-mimicking compounds have been termed xenoestrogens. Both estradiol and xenoestrogens can bind two estrogen receptors (ERs), ER alpha and ER beta, to elicit biological responses. The receptors are ligand inducible transcription factors that exhibit unique biological actions. While estradiol binds both receptors equally, some xenoestrogens have been shown to bind ER beta preferentially. When the ER is bound, the ligand induces a unique ER shape and in turn causes an array of tissue-specific biological responses. For example, the ligand tamoxifen, a commonly used breast cancer pharmaceutical, exhibits an ER antagonist response in the breast and an ER agonist response in the bone. This dual ligand quality characterizes what is now known as a selective estrogen receptor modulator (SERM). Peptide profiling, a novel ER ligand screening assay, is a method that can potentially identify SERMS by correlating in vitro ER conformation with in vivo biological response. Each ligand is screened using a two-hybrid fusion protein reporter gene assay. Upon ligand binding, the ER assumes a conformation; with this induced shape, some ER-interacting peptides will be able to bind while others will not. After screening a ligand against a library of fifteen different peptides, a unique peptide profile will figuratively illustrate the induced ER conformation. Eight xenoestrogens were screened in this experiment: estradiol, a natural physiological estrogen; resveratrol and genistein, two phytoestrogens; MPP, bisphenol A, and 4-hydroxytamoxifen, all synthetic estrogens; α-endosulfan and methoxychlor, both insecticides used on crops. Each ligand was found to have a unique peptide profile and, implicitly, a distinct ER conformation. The next step will be to determine each ligand's tissue specific activity and identify the unique peptide fingerprint that predicts its in vivo biological response. By correlating a ligand's tissue specific estrogenic activity with its unique ER conformation, peptide profiling will not only further elucidate tissue-specific ER activity differences but could also be used as a high-throughput screening tool for other potential environmental xenoestrogens and identify novel therapeutic SERMs.eng
dc.description.sponsorshipLife Sciences Undergraduate Research Opportunity Programeng
dc.identifier.urihttp://hdl.handle.net/10355/1992eng
dc.publisherUniversity of Missouri--Columbia. Office of Undergraduate Researcheng
dc.relation.ispartof2008 Summer Undergraduate Research and Creative Achievements Forum (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forumeng
dc.subjectpeptide profilingeng
dc.subjectestradioleng
dc.titlePeptide profiling: Correlating estrogen receptor conformation with biological responseeng
dc.typePresentationeng


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