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dc.contributor.authorBautista, Roderickeng
dc.contributor.authorHardaway, John C., 1978-eng
dc.contributor.authorZaghouani, Habibeng
dc.contributor.corporatenameUniversity of Missouri-Columbia. Office of Undergraduate Researcheng
dc.contributor.meetingnameSummer Undergraduate Research and Creative Achievements Forum (2005 : University of Missouri--Columbia)eng
dc.date2005eng
dc.date.issued2005eng
dc.descriptionAbstract only availableeng
dc.description.abstractA recent publication from our lab has provided evidence for the involvement of the α1 chain of the Interleukin-13 cytokine receptor (IL-13Rα1) in the development of the neonatal immune system. Specifically, we have shown that cell death of T helper type 1 (Th1) effector cells can be prevented by antibody-mediated blockade of IL-13Rα1. Currently, a knockout mouse deficient in expression of IL-13Rα1 is not available and the development of an IL-13Rα1 knockout mouse will provide new insights on the relationship between IL-13Rα1 signaling and neonatal immunity. In this effort we have begun construction of a targeting vector that will bear sufficient homology to the IL-13Rα1 wild-type locus to allow for deletion of exons 7, 8, and 9 via homologous recombination, thereby rendering that allele non-functional. After construction of the targeting vector, a collaborative effort between the Transgenic Animal Core facility here at the University of Missouri will continue throughout the remainder of the cell culture, embryonic manipulation, and screening processes.eng
dc.description.sponsorshipLife Sciences Fellowships/Meyerhoff Scholars Programeng
dc.identifier.urihttp://hdl.handle.net/10355/2056eng
dc.languageen_USeng
dc.publisherUniversity of Missouri--Columbia. Office of Undergraduate Researcheng
dc.relation.ispartof2005 Summer Undergraduate Research and Creative Achievements Forum (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Office of Undergraduate Research. Undergraduate Research and Creative Achievements Forumeng
dc.source.urihttp://undergradresearch.missouri.edu/forums-conferences/abstracts/abstract-detail.php?abstractid=eng
dc.subjectneonatal immune systemeng
dc.subjectinterleukin-13 cytokine receptoreng
dc.subjectIL-13Rα1 signalingeng
dc.subjectknockout targeting vectoreng
dc.titleConstruction of a knockout targeting vector to generate an Interleukin-13 Receptor α1 deficient Balb/c mouseeng
dc.typePresentationeng


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