A century later another surprise: A non-visual behavioral function of the white gene
Metadata[+] Show full item record
Discovery of the white mutation in Drosophila melanogaster has broadly influenced our understanding of the mechanisms of inheritance. We recently discovered a role of the white gene in memory formation. Thus, the white gene continues to provide insight into basic biological functions. We use two conditioning methods to routinely measure learning and memory in D. melanogaster, the heat-box, and classical olfactory conditioning. In the heat box experiments, white mutant flies' learning performance was notably impaired. However, in olfactory conditioning studies the mutant flies performed the same or better than wild-type flies. This differentiates the molecular mechanisms that support these conditioned behaviors. To better understand the regulatory elements that control white expression, we have initiated a molecular characterization of the white genomic locus. We identified the necessary regulatory elements by defining the deletion in the w1118 null allele. Using PCR methods we found that the deletion is about 7 kb long, and includes 5' regions, exon 1, and part of the first intron. Experiments to determine the sufficient set of regulatory elements for conditioned behavior were initiated. Two results argue that existing genomic transgenes do not contain all regulatory elements. First, mutations that affect eye color have molecular lesions outside a 14 kb genomic transgene. Second, attempted behavioral rescue experiments with this transgene fail. We interpret the failure of the 14 kb transgene to rescue as a consequence of incorrect white expression. Thus, we are creating a genomic construct that is 18 kb long that includes genomic DNA up to the next known gene. These approaches should define the regulatory regions necessary and sufficient for behaviorally important white expression.
Showing items related by title, author, creator and subject.
Heller, Jennifer (University of Missouri--Columbia, 2007)One job of social scientists is to parse out partial knowledge from every relevant social location in order to present a structural interpretation of social phenomena. A textual and content analysis was performed on both ...
Johnson, Martenzie (University of Missouri--Columbia[University of Missouri--Columbia], 2014)An experiment showed that a sports news article based on negative racial stereotypes about Black or White athletes can impede athletic performance based on the theory of stereotype threat. In the experiment, White participants ...
A qualitative examination of the support systems impacting the African American student persistence, retention and graduation at a predominantly white, research-extensive, land-grant Midwestern university Thorn, Elijah Andre ([University of Missouri--Columbia], 2014)Completion of a college degree serves as an necessity for many individuals who want to transform their lives, change their socio-economic status and have a better chance of living the "American Dream." For African Americans, ...