Browsing Division of Food Systems and Bioengineering (MU) by Thesis Advisor "Gillis, Kevin D."
Now showing items 1-5 of 5
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Automated detection of amperometric spikes resulting from quantal exocytosis and estimation of spike and pre-spike foot signal parameters
(University of Missouri--Columbia, 2018)Electrochemical microelectrodes can detect single-vesicle release events as "spikes" of amperometric current. We developed a template based "matched-filter" approach that performs least squares fit of a library of templates ... -
Integration of single-cell electropermeabilization together with electrochemical measurement of quantal exocytosis on microchips
(University of Missouri--Columbia, 2013)An electrochemical microelectrode located immediately adjacent to a single neuroendocrine cell can record spikes of amperometric current that result from quantal exocytosis of oxidizable transmitter from individual vesicles. ... -
Nano- and micro-scale studies of exocytosis
(University of Missouri--Columbia, 2007)Neurons and neuroendocrine cells contain vesicles packed with hormones or neurotransmitters. Upon appropriate stimulation, a rise in intracellular Ca2+ concentration triggers the fusion of vesicles with the outer membrane ... -
Optical stimulation of quantal exocytosis on transparent microchips
(University of Missouri--Columbia, 2007)Photorelease of caged Ca²⁺ is a uniquely powerful tool to study the dynamics of Ca²⁺-triggered exocytosis from individual cells. Using photolithography and other microfabrication techniques, we have developed transparent ... -
Study of low noise high throughput microchip device for electrochemical measurement from single cells
(University of Missouri--Columbia, 2012)We are developing transparent multi- electrochemical electrode arrays on microchips in order to automate measurement of quantal exocytosis of oxidizable transmitter from individual vesicles. In order to achieve low noise ...