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    Establishment of a protein reference map for soybean root hair cells

    Brechenmacher, Laurent
    Lee, Joohyun
    Sachdev, Sherri
    Song, Zhao, 1978-
    Nguyen, Tran Hong Nha
    Joshi, Trupti, 1977-
    Oehrle, Nathan Wayne, 1972-
    Libault, Marc
    Mooney, Brian P.
    Xu, Dong, 1965-
    Cooper, Bret
    Stacey, Gary, 1951-
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    [PDF] EstablishmentProteinReferenceMap.pdf (597.9Kb)
    Date
    2008
    Format
    Article
    Metadata
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    Abstract
    Root hairs are single tubular cells formed from the differentiation of epidermal cells on roots. They are involved in water and nutrient uptake and represent the infection site on leguminous roots by rhizobia, soil bacteria that establish a nitrogen-fixing symbiosis. Root hairs develop by polar cell expansion or tip growth, a unique mode of plant growth shared only with pollen tubes. A more complete characterization of root hair cell biology will lead to a better understanding of tip growth, the rhizobial infection process, and also lead to improvements in plant water and nutrient uptake. We analyzed the proteome of isolated soybean (Glycine max) root hair cells using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and shotgun proteomics (1D-PAGE-liquid chromatography and multidimensional protein identification technology) approaches. Soybean was selected for this study due to its agronomic importance and its root size. The resulting soybean root hair proteome reference map identified 1,492 different proteins. 2D-PAGE followed by mass spectrometry identified 527 proteins from total cell contents. A complementary shotgun analysis identified 1,134 total proteins, including 443 proteins that were specific to the microsomal fraction. Only 169 proteins were identified by the 2D-PAGE and shotgun methods, which highlights the advantage of using both methods. The proteins identified are involved not only in basic cell metabolism but also in functions more specific to the single root hair cell, including water and nutrient uptake, vesicle trafficking, and hormone and secondary metabolism. The data presented provide useful insight into the metabolic activities of a single, differentiated plant cell type.
    URI
    http://hdl.handle.net/10355/3241
    Part of
    Proteomics Center publications (MU)
    Citation
    Plant Physiology 149:670-682 (2009)
    Rights
    OpenAccess
    This work is licensed under a Creative Commons Attribution-NonCommerical-NoDerivs 3.0 License.
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    • Proteomics Center publications (MU)

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