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dc.contributor.authorStanley, David W.eng
dc.contributor.authorGoodman, Cynthiaeng
dc.contributor.authorAn, Shihengeng
dc.contributor.authorMcIntosh, Arthur H.eng
dc.contributor.authorSong, Qishengeng
dc.date.issued2007eng
dc.description.abstractProstaglandins (PGs) and other eicosanoids exert important physiological actions in insects and other invertebrates, including influencing ion transport and mediating cellular immune defense functions. Although these actions are very well documented, we have no information on the mechanisms of PGs actions in insect cells. Here we report on the outcomes of experiments designed to test our hypothesis that PGs modulate gene expression in an insect cell line established from pupal ovarian tissue of the moth Helicoverpa zea (BCIRL-HzAM1 cells). We treated cells with either PGA1 or PGE1 for 12 or 24 h then analyzed cell lysates by 2-D electrophoresis. Analysis of the gels by densitometry revealed substantial changes in protein expression in some of the protein spots we analyzed. These spots were processed for mass spectrometric analysis by MALDI TOF/TOF, which yielded in silico protein identities for all 34 spots. The apparent changes in three of the proteins were confirmed by semi-quantative PCR, showing that the changes in mRNA expression were reflected in changes in protein expression. The 34 proteins were sorted into six categories, protein actions, lipid metabolism, signal transduction, protection, cell functions and metabolism. The findings support the hypothesis that one mechanism of PG action in insect cells is the modulation of gene expression.eng
dc.identifier.citationInsect Biochemistry and Molecular Biology Vol. 38, No. 3, March 2008, pp 275-284eng
dc.identifier.issn0965-1748eng
dc.identifier.urihttp://hdl.handle.net/10355/3245eng
dc.languageEnglisheng
dc.publisherElseviereng
dc.relation.ispartofProteomics Center publications (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Christopher S. Bond Life Sciences Center. Proteomics Centereng
dc.rightsOpenAccesseng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommerical-NoDerivs 3.0 License.
dc.subjectInsect cell lineeng
dc.subjectAMHz1 cellseng
dc.subject2D electrophoresiseng
dc.subjectMass spectrometryeng
dc.subjectSemi-quantitative PCReng
dc.subject.disciplineLife scienceseng
dc.subject.lcshInsect cell biotechnologyeng
dc.subject.lcshTwo-dimensional electrophoresiseng
dc.subject.lcshMass spectrometryeng
dc.subject.lcshProstaglandinseng
dc.subject.lcshSemi-quantitative PCReng
dc.titleProstaglandins A1 and E1 influence gene expression in an established insect cell line (BCIRL-HzAM1 cells)eng
dc.typeArticleeng


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