Proteomics of canine lymphoma identifies potential cancer-specific protein markers

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Proteomics of canine lymphoma identifies potential cancer-specific protein markers

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/3255

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Title: Proteomics of canine lymphoma identifies potential cancer-specific protein markers
Author: McCaw, Dudley L.; Chan, Arvan S.; Stegner, Andrew L.; Mooney, Brian P.; Bryan, Jeffrey N.; Turnquist, Susan E.; Henry, Carolyn J.; Alexander, Hannah, 1947-; Alexander, Stephen, 1948-
Keywords: cancer biomarkers
mass spectrometry
macrophage capping protein
triosephosphate isomerase
glutathione S-transferase
Date: 2007-04-15
Publisher: American Association for Cancer Research
Citation: Clinical Cancer Research April 15, 2007 13, 2496
Abstract: Purpose: Early diagnosis of cancer is crucial for the success of treatment of the disease, and there is a need for markers whose differential expression between disease and normal tissue could be used as a diagnostic tool. Spontaneously occurring malignancies in pets provide a logical tool for translational research for human oncology. Lymphoma, one of the most common neoplasms in dogs, is similar to human non-Hodgkin's lymphoma and could serve as an experimental model system. Experimental Design: Thirteen lymph nodes from normal dogs and 11 lymph nodes from dogs with B-cell lymphoma were subjected to proteomic analysis using two-dimensional PAGE separation and matrix-assisted laser desorption/ionization time-of-flight analysis. Results: A total of 93 differentially expressed spots was subjected to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry analysis, and several proteins that showed differential expression were identified. Of these, prolidase (proline dipeptidase), triosephosphate isomerase, and glutathione S-transferase were down-regulated in lymphoma samples, whereas macrophage capping protein was up-regulated in the lymphoma samples. Conclusions: These proteins represent potential markers for the diagnosis of lymphoma and should be further investigated in human samples for validation of their utility as diagnostic markers.
URI: http://hdl.handle.net/10355/3255
ISSN: 1557-3265

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