dc.contributor.author | McCaw, Dudley L. | eng |
dc.contributor.author | Chan, Arvan S. | eng |
dc.contributor.author | Stegner, Andrew L. | eng |
dc.contributor.author | Mooney, Brian P. | eng |
dc.contributor.author | Bryan, Jeffrey N. | eng |
dc.contributor.author | Turnquist, Susan E. | eng |
dc.contributor.author | Henry, Carolyn J. | eng |
dc.contributor.author | Alexander, Hannah, 1947- | eng |
dc.contributor.author | Alexander, Stephen, 1948- | eng |
dc.date.issued | 2007 | eng |
dc.description.abstract | Purpose: Early diagnosis of cancer is crucial for the success of treatment of the disease, and there is a need for markers whose differential expression between disease and normal tissue could be used as a diagnostic tool. Spontaneously occurring malignancies in pets provide a logical tool for translational research for human oncology. Lymphoma, one of the most common neoplasms in dogs, is similar to human non-Hodgkin's lymphoma and could serve as an experimental model system. Experimental Design: Thirteen lymph nodes from normal dogs and 11 lymph nodes from dogs with B-cell lymphoma were subjected to proteomic analysis using two-dimensional PAGE separation and matrix-assisted laser desorption/ionization time-of-flight analysis. Results: A total of 93 differentially expressed spots was subjected to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry analysis, and several proteins that showed differential expression were identified. Of these, prolidase (proline dipeptidase), triosephosphate isomerase, and glutathione S-transferase were down-regulated in lymphoma samples, whereas macrophage capping protein was up-regulated in the lymphoma samples. Conclusions: These proteins represent potential markers for the diagnosis of lymphoma and should be further investigated in human samples for validation of their utility as diagnostic markers. | eng |
dc.identifier.citation | Clinical Cancer Research April 15, 2007 13, 2496 | eng |
dc.identifier.issn | 1557-3265 | eng |
dc.identifier.uri | http://hdl.handle.net/10355/3255 | eng |
dc.language | English | eng |
dc.publisher | American Association for Cancer Research | eng |
dc.relation.ispartof | Proteomics Center publications (MU) | eng |
dc.relation.ispartofcommunity | University of Missouri-Columbia. Christopher S. Bond Life Sciences Center. Proteomics Center | eng |
dc.rights | OpenAccess | eng |
dc.rights.license | This work is licensed under a Creative Commons Attribution-NonCommerical-NoDerivs 3.0 License. | |
dc.subject | cancer biomarkers | eng |
dc.subject | mass spectrometry | eng |
dc.subject | macrophage capping protein | eng |
dc.subject | triosephosphate isomerase | eng |
dc.subject | glutathione S-transferase | eng |
dc.subject.discipline | Life sciences | eng |
dc.subject.lcsh | Tumor markers | eng |
dc.subject.lcsh | Mass spectrometry | eng |
dc.subject.lcsh | Triose-phosphate isomerase | eng |
dc.subject.lcsh | Glutathione transferase | eng |
dc.title | Proteomics of canine lymphoma identifies potential cancer-specific protein markers | eng |
dc.type | Article | eng |