Proteomic identification of PKC-mediated expression of 20E-induced protein in Drosophila melanogaster

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Proteomic identification of PKC-mediated expression of 20E-induced protein in Drosophila melanogaster

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/3256

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dc.contributor.author Sun, Yaning
dc.contributor.author An, Shiheng
dc.contributor.author Henrich, Vincent C.
dc.contributor.author Sun, Xiaoping
dc.contributor.author Song, Qisheng
dc.date.accessioned 2009-10-28T14:14:22Z
dc.date.available 2009-10-28T14:14:22Z
dc.date.issued 2007-10-09
dc.identifier.citation Journal of Proteome Research, 2007, 6 (11), pp 4478-4488 en
dc.identifier.issn 1535-3907
dc.identifier.uri http://hdl.handle.net/10355/3256
dc.description.abstract Ecdysone receptor (EcR) and its heterodimeric partner, ultraspiracle protein (USP), are nuclear receptors that mediate the action of the insect molting hormone 20-hydroxyecdysone (20E). There is evidence that the activity of both receptors is affected by phosphorylation. Using a proteomic approach, we have shown that protein kinase C (PKC) activity is necessary for mediating 20E-induced expression of 14 specific proteins, including three previously reported 20E responsive proteins, and is also responsible for the intracellular localization of EcR and USP in larval salivary glands of Drosophila melanogaster. The 20E-dependent expression of the proteins was verified using real-time PCR and/or Western blot analysis. For some genes, inhibition of PKC activity reduced 20E-dependent transcriptional activity rapidly, raising the possibility that these are direct gene targets of EcR and USP. The data further indicate that PKC-mediated phosphorylation is also required for genes regulated indirectly by 20E-induced changes in the larval salivary gland. en
dc.language.iso en_US en
dc.publisher ACS en
dc.relation.ispartof Proteomics Center publications (MU) en
dc.subject nuclear receptor en
dc.subject protein expression en
dc.subject protein kinase en
dc.subject inhibitor en
dc.subject.lcsh Nuclear receptors (Biochemistry) en
dc.subject.lcsh Proteomics en
dc.subject.lcsh Protein kinase C en
dc.subject.lcsh Drosophila melanogaster en
dc.title Proteomic identification of PKC-mediated expression of 20E-induced protein in Drosophila melanogaster en
dc.type Article en
dc.subject.discipline Life sciences
dc.relation.ispartofcommunity University of Missouri-Columbia. Christopher S. Bond Life Sciences Center. Proteomics Center


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