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dc.contributor.authorSun, Yaning, 1978-eng
dc.contributor.authorAn, Shihengeng
dc.contributor.authorHenrich, Vincent C.eng
dc.contributor.authorSun, Xiaopingeng
dc.contributor.authorSong, Qishengeng
dc.date.issued2007-10eng
dc.description.abstractEcdysone receptor (EcR) and its heterodimeric partner, ultraspiracle protein (USP), are nuclear receptors that mediate the action of the insect molting hormone 20-hydroxyecdysone (20E). There is evidence that the activity of both receptors is affected by phosphorylation. Using a proteomic approach, we have shown that protein kinase C (PKC) activity is necessary for mediating 20E-induced expression of 14 specific proteins, including three previously reported 20E responsive proteins, and is also responsible for the intracellular localization of EcR and USP in larval salivary glands of Drosophila melanogaster. The 20E-dependent expression of the proteins was verified using real-time PCR and/or Western blot analysis. For some genes, inhibition of PKC activity reduced 20E-dependent transcriptional activity rapidly, raising the possibility that these are direct gene targets of EcR and USP. The data further indicate that PKC-mediated phosphorylation is also required for genes regulated indirectly by 20E-induced changes in the larval salivary gland.eng
dc.identifier.citationJournal of Proteome Research, 2007, 6 (11), pp 4478-4488eng
dc.identifier.issn1535-3907eng
dc.identifier.urihttp://hdl.handle.net/10355/3256eng
dc.publisherACSeng
dc.relation.ispartofProteomics Center publications (MU)eng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Christopher S. Bond Life Sciences Center. Proteomics Centereng
dc.subjectnuclear receptoreng
dc.subjectprotein expressioneng
dc.subjectprotein kinaseeng
dc.subjectinhibitoreng
dc.subject.disciplineLife scienceseng
dc.subject.lcshNuclear receptors (Biochemistry)eng
dc.subject.lcshProteomicseng
dc.subject.lcshProtein kinase Ceng
dc.subject.lcshDrosophila melanogastereng
dc.titleProteomic identification of PKC-mediated expression of 20E-induced protein in Drosophila melanogastereng
dc.typeArticleeng


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