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    Evaluation of a new fluorescence quantitative PCR test for diagnosing Helicobacter pylori infection in children

    Ou, Zhiying
    Xiong, Liya
    Li, Ding-You
    Geng, Lanlan
    Li, Lixia
    Chen, Peiyu
    Yang, Min
    Zeng, Yongmei
    Zhou, Zhenwen
    Xia, Huimin
    Gong, Sitang
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    [XML] 1471-230X-13-7.xml (55.56Kb)
    [PDF] 1471-230X-13-7.pdf (216.0Kb)
    Date
    2013-01-14
    Format
    Journal Article
    Metadata
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    Abstract
    Abstract Background Numerous diagnostic tests are available to detect Helicobactor pylori (H. pylori). There has been no single test available to detect H. pylori infection reliably. We evaluated the accuracy of a new fluorescence quantitative PCR (fqPCR) for H. pylori detection in children. Methods Gastric biopsy specimens from 138 children with gastritis were sent for routine histology exam, rapid urease test (RUT) and fqPCR. 13C-urea breath test (13C-UBT) was carried out prior to endoscopic procedure. Gastric fluids and dental plaques were also collected for fqPCR analysis. Results 38 children (27.5%) were considered positive for H. pylori infection by gold standard (concordant positive results on 2 or more tests). The remaining 100 children (72.5%) were considered negative for H. pylori. Gastric mucosa fqPCR not only detected all 38 H. pylori positive patients but also detected 8 (8%) of the 100 gold standard-negative children or 11 (10.7%) of the 103 routine histology-negative samples. Therefore, gastric mucosa fqPCR identified 46 children (33.3%) with H. pylori infection, significantly higher than gold standard or routine histology (P<0.01). Both gastric fluid and dental plaque fqPCR only detected 32 (23.2%) and 30 (21.7%) children with H. pylori infection respectively and was significantly less sensitive than mucosa fqPCR (P<0.05) but was as sensitive as non-invasive UBT. Conclusions Gastric mucosa fqPCR was more sensitive than routine histology, RUT, 13C-UBT alone or in combination to detect H. pylori infection in children with chronic gastritis. Either gastric fluid or dental plaque PCR is as reliable as 13C-UBT for H. pylori detection.
    URI
    http://dx.doi.org/10.1186/1471-230X-13-7
    http://hdl.handle.net/10355/33119
    Citation
    BMC Gastroenterology. 2013 Jan 14;13(1):7
    Rights
    Zhiying Ou et al.; licensee BioMed Central Ltd.
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    • BioMed Central Open Access Articles (UMKC)

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