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dc.contributor.authorWan, Jinrong
dc.contributor.authorTorres, Michael
dc.contributor.authorGanapathy, Ashwin
dc.contributor.authorThelen, Jay J.
dc.contributor.authorDaGue, Beverly B.
dc.contributor.authorMooney, Brian P.
dc.contributor.authorXu, Dong, 1965-
dc.contributor.authorStacey, Gary, 1951-
dc.date.issued2005-01eng
dc.descriptionhttp://www.soyroothair.org/publications.phpen
dc.description.abstractInfection of soybean root hairs by Bradyrhizobium japonicum is the first of several complex events leading to nodulation. In the current proteomic study, soybean root hairs after inoculation with B. japonicum were separated from roots. Total proteins were analyzed by two-dimensional (2-D) polyacrylamide gel electrophoresis. In one experiment, 96 protein spots were analyzed by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) to compare protein profiles between uninoculated roots and root hairs. Another 37 spots, derived from inoculated root hairs over different timepoints, were also analyzed by tandem MS (MS/MS). As expected, some proteins were differentially expressed in root hairs compared with roots (e.g., a chitinase and phosphoenolpyruvate carboxylase). Out of 37 spots analyzed by MS/MS, 27 candidate proteins were identified by database comparisons. These included several proteins known to respond to rhizobial inoculation (e.g., peroxidase and phenylalanine-ammonia lyase). However, novel proteins were also identified (e.g., phospholipase D and phosphoglucomutase). This research establishes an excellent system for the study of root-hair infection by rhizobia and, in a more general sense, the functional genomics of a single, plant cell type. The results obtained also indicate that proteomic studies with soybean, lacking a complete genome sequence, are practical.en
dc.description.sponsorshipThe project was funded by a joint University of Missouri-Monsanto grant to G. Stacey.en
dc.identifier.citationMPMI Vol. 18, No. 5, 2005, pp. 458-467. DOI: 10.1094/MPMI -18-0458.en
dc.identifier.issn0894-0282
dc.identifier.urihttp://hdl.handle.net/10355/3478
dc.publisherAPS Pressen
dc.relation.ispartofFunctional Genomics of Root Hair Infection project publications (MU)
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. National Center for Soybean Biotechnology. Functional Genomics of Root Hair Infection project
dc.subjectrhizobiumen
dc.subject.lcshLegumesen
dc.subject.lcshSymbiosisen
dc.subject.lcshRhizobiumen
dc.titleProteomic Analysis of Soybean Root Hairs After Infection by Bradyrhizobium japonicumen
dc.typeArticleen


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