Proteomic Analysis of Soybean Root Hairs After Infection by Bradyrhizobium japonicum

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Proteomic Analysis of Soybean Root Hairs After Infection by Bradyrhizobium japonicum

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[-] show simple item record Wan, Jinrong Torres, Michael Ganapathy, Ashwin Thelen, Jay J. DaGue, Beverly B. Mooney, Brian P. Xu, Dong, 1965- Stacey, Gary, 1951- 2009-11-04T20:17:32Z 2009-11-04T20:17:32Z 2005-01 eng
dc.identifier.citation MPMI Vol. 18, No. 5, 2005, pp. 458-467. DOI: 10.1094/MPMI -18-0458. en
dc.identifier.issn 0894-0282
dc.description en
dc.description.abstract Infection of soybean root hairs by Bradyrhizobium japonicum is the first of several complex events leading to nodulation. In the current proteomic study, soybean root hairs after inoculation with B. japonicum were separated from roots. Total proteins were analyzed by two-dimensional (2-D) polyacrylamide gel electrophoresis. In one experiment, 96 protein spots were analyzed by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) to compare protein profiles between uninoculated roots and root hairs. Another 37 spots, derived from inoculated root hairs over different timepoints, were also analyzed by tandem MS (MS/MS). As expected, some proteins were differentially expressed in root hairs compared with roots (e.g., a chitinase and phosphoenolpyruvate carboxylase). Out of 37 spots analyzed by MS/MS, 27 candidate proteins were identified by database comparisons. These included several proteins known to respond to rhizobial inoculation (e.g., peroxidase and phenylalanine-ammonia lyase). However, novel proteins were also identified (e.g., phospholipase D and phosphoglucomutase). This research establishes an excellent system for the study of root-hair infection by rhizobia and, in a more general sense, the functional genomics of a single, plant cell type. The results obtained also indicate that proteomic studies with soybean, lacking a complete genome sequence, are practical. en
dc.description.sponsorship The project was funded by a joint University of Missouri-Monsanto grant to G. Stacey. en
dc.language.iso en_US en
dc.publisher APS Press en
dc.relation.ispartof Functional Genomics of Root Hair Infection project publications (MU)
dc.subject rhizobium en
dc.subject.lcsh Legumes en
dc.subject.lcsh Symbiosis en
dc.subject.lcsh Rhizobium en
dc.title Proteomic Analysis of Soybean Root Hairs After Infection by Bradyrhizobium japonicum en
dc.type Article en
dc.relation.ispartofcommunity University of Missouri-Columbia. National Center for Soybean Biotechnology. Functional Genomics of Root Hair Infection project

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