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dc.contributor.advisorAnderson, Deborah M., 1969-eng
dc.contributor.authorEisele, Nicholas A.eng
dc.date.issued2012eng
dc.date.submitted2012 Springeng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on May 13, 2013).eng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionDissertation advisor: Dr. Deborah M. Andersoneng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionVita.eng
dc.descriptionPh. D. University of Missouri-Columbia 2012.eng
dc.description"May 2012"eng
dc.description.abstractYersinia pestis is the etiologic agent of plague and is responsible for more human deaths throughout history than any other bacterial pathogen. During infection bacteria inject effector proteins into target host cells using a Type III secretion system (T3SS). Immunity to plague is conferred to the host via antibodies targeting LcrV, an essential component of the T3SS. Although protective anti-LcrV antibodies block injection, the precise mechanism of protection is unknown. As such, we sought here to define the requirements for humoral immunity to plague. We found that protective antibodies not only block T3S, but also opsonize bacteria for phagocytic uptake. Thus, we next examined the role of macrophages in disease clearance and found that while cells limit bacterial replication, they are unable to clear infection. Thus we hypothesized that another immune cell is important for disease clearance and found that recruitment and activation of neutrophils is essential for clearing infection in the presence of antibodies. Together, the data support a model whereby protective antibodies block T3S injection while simultaneously opsonizing bacteria for phagocytic uptake. However, although macrophages limit bacterial replication, cells are unable to kill organisms and rely on neutrophils to clear the infection.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.format.extentxii, 160 pageseng
dc.identifier.oclc864760237eng
dc.identifier.urihttps://hdl.handle.net/10355/35151
dc.identifier.urihttps://doi.org/10.32469/10355/35151eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertations.eng
dc.rightsOpenAccesseng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.subjectbacterial pathogeneng
dc.subjectdisease clearanceeng
dc.subjectneutrophilseng
dc.titleFactors contributing to humoral immunity against pneumonic plagueeng
dc.typeThesiseng
thesis.degree.disciplineMicrobiology (Medicine) (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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