dc.contributor.advisor | Green, Jonathan A., 1969- | eng |
dc.contributor.author | Lichtenauer, Anna Marie | eng |
dc.date.issued | 2013 | eng |
dc.date.submitted | 2013 Spring | eng |
dc.description | Title from PDF of title page (University of Missouri--Columbia, viewed on September 13, 2013). | eng |
dc.description | The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. | eng |
dc.description | Thesis advisor: Dr. Jonathan A. Green | eng |
dc.description | Includes bibliographical references. | eng |
dc.description | M.S. University of Missouri--Columbia 2013. | eng |
dc.description | Dissertations, Academic -- University of Missouri--Columbia -- Animal sciences. | eng |
dc.description | "May 2013" | eng |
dc.description.abstract | [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] The cell permeable cysteine peptidase inhibitor, E-64, has shown utility as a potent and irreversible inhibitor for many cysteine proteases. Consequently, it has been studied in several model systems, including ones involving oocyte and embryo culture. In cattle, administration of E-64 during oocyte IVM or embryo culture resulted in improvements in both oocyte maturation and embryo development. In this study the effects of E-64 were investigated in a porcine IVM/IVF system. Significantly increased rates of oocyte maturation were recorded for COCs treated with E-64. In addition, an increase in cleavage rates was found. However, blastocyst development was not altered. Furthermore, in vitro maturation of porcine COCs using E-64 was performed with COCs segregated into morphologically distinct developmental groups. Those COCs defined as 'good' matured in significantly higher proportions when E-64 was added to the in vitro maturation medium compared to 'good' COCs in the absence of E-64. In addition, 'good' COCs treated with E-64 produced blastocysts with numerically higher total cell counts. In vitro matured COC and oocyte mRNA was extracted and compared to in vivo derived oocyte mRNA. There was a significant difference in cathepsin K mRNA abundance between in vitro matured oocytes and in vivo produced oocytes. However, there was no significant difference in cathepsin B mRNA between the samples, despite that such a difference had been expected based on previous reports in bovine oocytes. In vitro maturation with cathepsin B and cathepsin K specific inhibitors produced oocytes that reached MII at percentages consistent with those of untreated oocytes. Protein expression was used to determine if cathepsin B was being affected at the protein level. Western blots revealed cathepsin B in COCs, but not in denuded oocytes. Additionally, immunocytochemistry verified these results by showing cathepsin B localization in cumulus cells but not in the denuded oocyte. | eng |
dc.format.extent | xii, 94 pages | eng |
dc.identifier.uri | http://hdl.handle.net/10355/38540 | |
dc.language | English | eng |
dc.publisher | University of Missouri--Columbia | eng |
dc.relation.ispartofcommunity | University of Missouri--Columbia. Graduate School. Theses and Dissertations | eng |
dc.rights | Access is limited to the campuses of the University of Missouri. | eng |
dc.source | Submitted by the University of Missouri--Columbia Graduate School | eng |
dc.subject | oocyte maturation | eng |
dc.subject | embryo development | eng |
dc.subject | cathepsin | eng |
dc.subject | cysteine protease | eng |
dc.title | The inhibition of cysteine peptidases improves in vitro maturation of porcine cumulus-oocyte-complexes | eng |
dc.type | Thesis | eng |
thesis.degree.discipline | Animal sciences (MU) | eng |
thesis.degree.grantor | University of Missouri--Columbia | eng |
thesis.degree.level | Masters | eng |
thesis.degree.name | M.S. | eng |