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dc.contributor.advisorSharma, Krishna K.eng
dc.contributor.authorKannan, Rama, 1975-eng
dc.contributor.otherUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2013 Dissertationseng
dc.date.issued2013eng
dc.date.submitted2013 Summereng
dc.description.abstract[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Age-related cataract is the result of aggregation of lens proteins, crystallins. The formation of aggregates in the aging lens has been shown to correlate with progressive accumulation of specific low molecular weight (LMW) peptides (<3.5-kDa) derived from crystallins. Prominent among LMW peptides is [alpha]A66-80, a peptide derived from [alpha]A-crystallin and present in increased concentrations in the water-insoluble fractions of the aging lens. We investigated the characteristics of the peptide and its interactions with soluble [alpha]-crystallin. The [alpha]A66-80 peptide has amyloid-like properties and preferentially insolubilizes [alpha]-crystallin from soluble lens fractions. The peptide interacts with [alpha]B-crystallin at multiple sites, suppresses [alpha]-crystallin chaperone activity, increases the surface hydrophobicity, decreases the solubility and induces its aggregation. A sequential proline scan studies shows those residues 71-77 critical for the activity of the peptide. Lens extracts exhibit protease activity that generates [alpha]A66-80. Thus, the [alpha]-crystallin-derived peptide could play a role in the pathogenesis of cataract formation.eng
dc.description.bibrefIncludes bibliographical references (pages 199-226).eng
dc.format.extent1 online resource (xvii, 227 pages) : illustrations (some color)eng
dc.identifier.oclc892849790eng
dc.identifier.urihttps://doi.org/10.32469/10355/40172eng
dc.identifier.urihttps://hdl.handle.net/10355/40172
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertationseng
dc.rightsAccess is limited to the campus of the University of Missouri--Columbia.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.sourceSubmitted by the University of Missouri--Columbia Graduate School.eng
dc.subject.lcshCataract in old age -- Pathogenesiseng
dc.subject.lcshPeptideseng
dc.subject.lcshProteins -- Biotechnologyeng
dc.subject.lcshAggregation (Chemistry)eng
dc.title[alpha]A-crystallin derived peptide, [alpha]A66-80 induced aggregation and precipitation of soluble [alpha]-crystallin : a contribution to age-related cataract formationeng
dc.typeThesiseng
thesis.degree.disciplineBiochemistry (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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