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dc.contributor.advisorRivera, Rocío Melissaeng
dc.contributor.authorNegrón Pérez, Verónica M., 1988-eng
dc.date.issued2013eng
dc.date.submitted2013 Summereng
dc.descriptionJuly 2013.eng
dc.descriptionA Thesis Presented to the Faculty of the Graduate School At the University of Missouri In Partial Fulfillment Of the Requirements for the Degree Master of Science.eng
dc.descriptionThesis supervisor: Dr. Rocio Melissa Rivera.eng
dc.descriptionIncludes vita.eng
dc.description.abstractH19 is a maternally-expressed imprinted non-coding RNA with tumor suppressor activity. H19 is located in an imprinted cluster which contains the paternally-expressed fetal growth factor Igf2. Monoallelic expression of H19 and Igf2 is regulated by allele-specific interaction of shared enhancers. Previous work has shown that embryo culture can result in biallelic expression of H19. However, preliminary data in our laboratory suggest that biallelic expression of H19 is a normally-occurring event during peri-implantation in the mouse. To test this, the allelic expression of H19 was compared between in vitro- and in vivo-developed embryos at 84, 96, and 108 hours following ovulation. We found that as embryos advance through preimplantation development they start expressing H19 in a biallelic manner regardless of treatment. In a subsequent study, we microdissected peri-implantation embryos into two halves, one containing the inner cell mass (ICM) and the other containing the primary trophoblast giant cells (PTGC). PTGCs are invasive embryonic cells that initiate uterine implantation. The PTGC-containing half of the embryo had greater expression of H19 and less Igf2 than the ICMcontaining half of the embryo. We speculate that biallelic expression of H19 is part of a normal physiologic event in peri-implantation mouse embryos to reduce Igf2 levels in the PTGCs in order to control their invasive behavior.eng
dc.description.bibrefIncludes bibliographical references (pages 98-138).eng
dc.format.extent1 online resource (xv, 151 pages) : illustrations (some color)eng
dc.identifier.oclc897471106eng
dc.identifier.urihttp://hdl.handle.net/10355/40195
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.source.originalSubmitted by the University of Missouri--Columbia Graduate Schooleng
dc.subject.lcshFetus -- Development -- Animal modelseng
dc.subject.lcshAntioncogenes -- Animal modelseng
dc.subject.lcshNon-coding RNA -- Animal modelseng
dc.subject.lcshGene expression -- Animal modelseng
dc.subject.lcshEmbryology, Experimentaleng
dc.titleDetermination of allelic expression of H19 in pre- and peri-implantation mouse embryoseng
dc.typeThesiseng
thesis.degree.disciplineAnimal sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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