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dc.contributor.advisorWall, Judy D.eng
dc.contributor.authorHemme, Christopher Lee, 1974-eng
dc.date.issued2004eng
dc.date.submitted2004 Falleng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file (viewed on June 29, 2006)eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionVita.eng
dc.descriptionThesis (Ph. D.) University of Missouri-Columbia 2004.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biochemistry (Agriculture)eng
dc.description.abstractThe sulfate-reducing bacteria are a morphologically diverse group of organisms characterized by the ability to couple the enzymatic reduction of sulfate to energy production and growth. This metabolic activity has profound economic and environmental consequences such as the corrosion of metal structures and the souring of petroleum reserves. The sulfate-reducing bacteria are also among a select group of organisms that may be used as tools for the bioremediation of toxic heavy metal contaminants from the environment. To understand the mechanisms through which these bacteria impact our environment both positively and negatively, genomic studies have been undertaken to predict the metabolic and regulatory networks of two species of the genus Desulfovibrio. Studies have focused on the elucidation of carbon metabolic pathways, the role of CRP-FNR proteins in the regulation of Desulfovibrio metabolic pathways, and the prediction of global regulatory networks using bioinformatics techniques. Surprisingly, several hexose metabolic genes were found despite the fact that biochemical evidence suggests that these bacteria do not use hexose sugars as growth substrates. This physiological paradox was explored. Secondary pathways for the metabolism of galactose and the synthesis of [alpha],[alpha]-trehalose were observed in Desulfovibrio desulfuricans G20 but not Desulfovibrio vulgaris Hildenborough.Physiological experiments showed that despite the presence of a complete set of galactose metabolism genes, Dv. desulfuricans was unable to utilize galactose as the sole carbon source for growth. Growth experiments using [14C]-labeled galactose in the presence of lactate suggested that galactose was incorporated into the cell. This result coupled with the published observation that the extracellular polymeric substances (EPS) of Dv. desulfuricans strains contained detectable levels of galactose suggests that metabolism of galactose occurs for the purposes of production of EPS and possibly lipopolysaccharide (LPS). To explore the possible hierarchical regulation of substrate utilization, global regulators responding to redox signals were sought. Sequencing revealed multiple orthologs of the CRP-FNR genes in Desulfovibrio. A mutant strain of Dv. vulgaris was constructed that was interrupted in a gene encoding a putative CRP-FNR protein. A phenotypic analysis of the mutant strain showed no significant differences in the growth rates and growth yields of the cells compared to wild type when grown on lactate-sulfate, pyruvate-sulfate (respiration), pyruvate alone (fermentation) or formate-sulfate. However, the mutant was shown to be impaired in growth on ethanol-sulfate compared to wild type, suggesting the involvement of this protein in either the cellular ability to use ethanol or maintain cellular integrity. A computational analysis of the promoter regions of putative transcription units of Desulfovibrio revealed possible regulatory protein binding motifs homologous to the E. coli CRP and GalR binding sites as well as a motif common to genes encoding phosphate homeostasis proteins. Further examination revealed a set of statistically significant motifs not immediately identified as E. coli homologs. This set of motifs may represent unique regulatory motifs of Desulfovibrio.eng
dc.identifier.merlinb55842550eng
dc.identifier.oclc70208794eng
dc.identifier.urihttps://hdl.handle.net/10355/4084
dc.identifier.urihttps://doi.org/10.32469/10355/4084eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.sourceSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subject.lcshDesulfovibrioeng
dc.subject.lcshSulfur bacteriaeng
dc.titleExamination of metabolic and regulatory networks of desulfovibrio specieseng
dc.typeThesiseng
thesis.degree.disciplineBiochemistry (Agriculture) (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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