dc.contributor.advisor | Beamer, Lesa J. (Lesa Jean) | eng |
dc.contributor.author | Regni, Catherine A., 1973- | eng |
dc.date.issued | 2005 | eng |
dc.date.submitted | 2005 Fall | eng |
dc.description | The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. | eng |
dc.description | Title from title screen of research.pdf file (viewed on October 18, 2007) | eng |
dc.description | Vita. | eng |
dc.description | Includes bibliographical references. | eng |
dc.description | Thesis (Ph. D.) University of Missouri-Columbia 2005. | eng |
dc.description | Dissertations, Academic -- University of Missouri--Columbia -- Biochemisty (Agriculture) | eng |
dc.description.abstract | The human pathogen Pseudomonas aeruginosa expresses a variety of cell surface polysaccharides, including alginate, lipopolysaccharide (LPS), and rhamnolipid. P. aeruginosa is the primary cause of chronic lung infections in cystic fibrosis (CF) patients, and these molecules contribute to its virulence. This dual-specificity enzyme phosphomannomutase/phosphoglucomutase (PMM/PGM) is required for the production of these molecules. The structure of PMM/PGM was previously solved in our laboratory, showing that the protein has four domains organized in a "heart shape," with the active site in a deep cleft formed by residues from each domain. Recently, we have determined the structures of PMM/PGM bound to its two substrates, two products, and an intermediate at 2.0 Å resolution or higher. These structures reveal the structural basis for diverse substrate recognition by the enzyme and pinpoint residues important for accomplishing the dynamic reorientation step of the reaction. | eng |
dc.identifier.merlin | b60595930 | eng |
dc.identifier.oclc | 174972669 | eng |
dc.identifier.uri | https://hdl.handle.net/10355/4134 | |
dc.identifier.uri | https://doi.org/10.32469/10355/4134 | eng |
dc.language | English | eng |
dc.publisher | University of Missouri--Columbia | eng |
dc.relation.ispartofcommunity | University of Missouri--Columbia. Graduate School. Theses and Dissertations | eng |
dc.source | Submitted by University of Missouri--Columbia Graduate School. | eng |
dc.subject | phosphomannomutase/phosphoglucomutase. | eng |
dc.subject | phosphomannomutase/phosphoglucomutase | eng |
dc.subject.lcsh | Pseudomonas aeruginosa infections | eng |
dc.subject.lcsh | Cystic fibrosis -- Patients | eng |
dc.title | Structural studies of PMM/PGM from Pseudomonas aeruginosa | eng |
dc.type | Thesis | eng |
thesis.degree.discipline | Biochemistry (Agriculture) (MU) | eng |
thesis.degree.grantor | University of Missouri--Columbia | eng |
thesis.degree.level | Doctoral | eng |
thesis.degree.name | Ph. D. | eng |