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dc.contributor.advisorBeamer, Lesa J. (Lesa Jean)en
dc.contributor.authorRegni, Catherine A., 1973-en_US
dc.date.issued2005eng
dc.date.submitted2005 Fallen
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.en_US
dc.descriptionTitle from title screen of research.pdf file (viewed on October 18, 2007)en_US
dc.descriptionVita.en_US
dc.descriptionIncludes bibliographical references.en_US
dc.descriptionThesis (Ph. D.) University of Missouri-Columbia 2005.en_US
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biochemisty (Agriculture)en_US
dc.description.abstractThe human pathogen Pseudomonas aeruginosa expresses a variety of cell surface polysaccharides, including alginate, lipopolysaccharide (LPS), and rhamnolipid. P. aeruginosa is the primary cause of chronic lung infections in cystic fibrosis (CF) patients, and these molecules contribute to its virulence. This dual-specificity enzyme phosphomannomutase/phosphoglucomutase (PMM/PGM) is required for the production of these molecules. The structure of PMM/PGM was previously solved in our laboratory, showing that the protein has four domains organized in a "heart shape," with the active site in a deep cleft formed by residues from each domain. Recently, we have determined the structures of PMM/PGM bound to its two substrates, two products, and an intermediate at 2.0 Å resolution or higher. These structures reveal the structural basis for diverse substrate recognition by the enzyme and pinpoint residues important for accomplishing the dynamic reorientation step of the reaction.en_US
dc.identifier.merlin.b60595930en_US
dc.identifier.oclc174972669en_US
dc.identifier.otherRegniC-121605-D3111en_US
dc.identifier.urihttp://hdl.handle.net/10355/4134
dc.publisherUniversity of Missouri--Columbiaen_US
dc.relation.ispartofcollection2005 Freely available dissertations (MU)
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2005 Dissertations
dc.subjectphosphomannomutase/phosphoglucomutase.en_US
dc.subjectphosphomannomutase/phosphoglucomutaseen_US
dc.subject.lcshPseudomonas aeruginosa infectionsen_US
dc.subject.lcshCystic fibrosis -- Patientsen_US
dc.titleStructural studies of PMM/PGM from Pseudomonas aeruginosaen_US
dc.typeThesisen_US
thesis.degree.disciplineBiochemistry (Agriculture)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoralen_US
thesis.degree.namePh. D.en_US


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