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dc.contributor.advisorBeamer, Lesa J. (Lesa Jean)eng
dc.contributor.authorRegni, Catherine A., 1973-eng
dc.date.issued2005eng
dc.date.submitted2005 Falleng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file (viewed on October 18, 2007)eng
dc.descriptionVita.eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionThesis (Ph. D.) University of Missouri-Columbia 2005.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biochemisty (Agriculture)eng
dc.description.abstractThe human pathogen Pseudomonas aeruginosa expresses a variety of cell surface polysaccharides, including alginate, lipopolysaccharide (LPS), and rhamnolipid. P. aeruginosa is the primary cause of chronic lung infections in cystic fibrosis (CF) patients, and these molecules contribute to its virulence. This dual-specificity enzyme phosphomannomutase/phosphoglucomutase (PMM/PGM) is required for the production of these molecules. The structure of PMM/PGM was previously solved in our laboratory, showing that the protein has four domains organized in a "heart shape," with the active site in a deep cleft formed by residues from each domain. Recently, we have determined the structures of PMM/PGM bound to its two substrates, two products, and an intermediate at 2.0 Å resolution or higher. These structures reveal the structural basis for diverse substrate recognition by the enzyme and pinpoint residues important for accomplishing the dynamic reorientation step of the reaction.eng
dc.identifier.merlin.b60595930eng
dc.identifier.oclc174972669eng
dc.identifier.otherRegniC-121605-D3111eng
dc.identifier.urihttp://hdl.handle.net/10355/4134eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.source.originalSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subjectphosphomannomutase/phosphoglucomutase.eng
dc.subjectphosphomannomutase/phosphoglucomutaseeng
dc.subject.lcshPseudomonas aeruginosa infectionseng
dc.subject.lcshCystic fibrosis -- Patientseng
dc.titleStructural studies of PMM/PGM from Pseudomonas aeruginosaeng
dc.typeThesiseng
thesis.degree.disciplineBiochemistry (Agriculture) (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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