Shared more. Cited more. Safe forever.
    • advanced search
    • submit works
    • about
    • help
    • contact us
    • login
    View Item 
    •   MOspace Home
    • University of Missouri-Columbia
    • Graduate School - MU Theses and Dissertations (MU)
    • Theses and Dissertations (MU)
    • Dissertations (MU)
    • 2010 Dissertations (MU)
    • 2010 MU dissertations - Freely available online
    • View Item
    •   MOspace Home
    • University of Missouri-Columbia
    • Graduate School - MU Theses and Dissertations (MU)
    • Theses and Dissertations (MU)
    • Dissertations (MU)
    • 2010 Dissertations (MU)
    • 2010 MU dissertations - Freely available online
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    advanced searchsubmit worksabouthelpcontact us

    Browse

    All of MOspaceCommunities & CollectionsDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis SemesterThis CollectionDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis Semester

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular AuthorsStatistics by Referrer

    Proteomic identification of the differentially expressed and phosphorylated proteins in 20-hydroxyecdysone (20E) signal transduction pathway in salivary gland of Drosophila melanogaster

    Sun, Yaning, 1978-
    View/Open
    [PDF] public.pdf (1.958Kb)
    [PDF] research.pdf (2.448Mb)
    [PDF] short.pdf (235.5Kb)
    Date
    2010
    Format
    Thesis
    Metadata
    [+] Show full item record
    Abstract
    Protein kinase C (PKC) plays important role in 20-hydroxyecdysone (20E) signal transduction, however, little is known about the exact role of PKC in this process. In my research, PKC-regulated phosphorylation in 20E signal transduction is investigated in the salivary gland of Drosophila melanogaster. Our experiments demonstrate that PKCregulated phosphorylation is responsible for the intracellular localization of ecdysone receptor (EcR) and its heterodimeric partner, ultraspiracle protein (USP), which is possibly due to the forming of receptor complex with chaperons. We also confirmed PKC-regulated phosphorylation is required in 20E induced protein expression and identified 14 proteins induced by 20E but inhibited by PKC inhibitor. Using 2D Western blot and phospho-(Ser) PKC substrate, we were able to identify four phosphorylated PKC substrates in 20E signal transduction process, which may function in 20E-induced gene transcription/translation process or in ecdysteroid transporting. In addition, PKC isoforms in the salivary gland were also investigated by RNA interference (RNAi). For the first time, we showed the successful application of RNAi technology by soaking the salivary glands of D. melanogaster with dsRNAs.
    URI
    https://hdl.handle.net/10355/41913
    https://doi.org/10.32469/10355/41913
    Degree
    Ph. D.
    Thesis Department
    Plant sciences (MU)
    Rights
    OpenAccess.
    This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
    Collections
    • 2010 MU dissertations - Freely available online
    • Plant Sciences electronic theses and dissertations (MU)

    Send Feedback
    hosted by University of Missouri Library Systems
     

     


    Send Feedback
    hosted by University of Missouri Library Systems