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dc.contributor.advisorNewton, Kathleen J.eng
dc.contributor.authorLangewisch, Tiffany, 1981-eng
dc.date.issued2012eng
dc.date.submitted2012 Falleng
dc.descriptionTitle from PDF of title page (University of Missouri--Columbia, viewed on April 23, 2014).eng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionVitaeng
dc.description.abstractMaize S-type cytoplasmic male sterility (CMS-S) is a maternally inherited trait that prevents pollen grains from developing to maturity. CMS-S is associated with the high levels of a novel mitochondrial transcript, orf355/orf77. Cleavage of this RNA, mediated by the nuclear restorer Rf3, reverses the sterility. Rf3 was previously mapped on the long arm of chromosome 2. The goals of this research were to fine-map the locus and to identify Rf3 using a candidate gene approach. Genotyping of nearisogenic lines (NILs) mapped Rf3 to a 1.98 Mb region of 2L. Six candidate genes, all predicted to code for mitochondrially targeted pentatricopeptide repeat proteins (PPR), were PCR-amplified, sequenced, and compared from multiple Rf3-containing NILs and non-restoring rf3 inbreds. One PPR-Rf3 candidate gene had two consistent differences between multiple restoring and non-restoring lines. Gene expression in pre-emergent tassels from the fertility-restored and non-restored plants was compared. Within the 3 Mb region surrounding Rf3, 9 genes were differentially expressed between restoring and non-restoring lines, including genes that could code for an ATP-binding protein, an ATPase, and four PPR proteins. Although Rf3 has not yet been identified, this study has revealed five promising candidates.eng
dc.format.extentxvii, 310 pageseng
dc.identifier.urihttp://hdl.handle.net/10355/41914
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.source.originalSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subjectCytoplasmic male sterilityeng
dc.subjectRestorer-of-fertilityeng
dc.subjectRNA-sequencingeng
dc.subjectPentatricopeptide repeat proteineng
dc.subject.FASTCytoplasmic male sterilityeng
dc.subject.FASTCorn -- Breedingeng
dc.subject.FASTAldehyde dehydrogenaseeng
dc.titleLocalization of the Rf3 restorer-of-fertility gene for maize S-type cytoplasmic male sterilityeng
dc.typeThesiseng
thesis.degree.disciplineBiological sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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