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dc.contributor.advisorWhite, Henryeng
dc.contributor.authorGraham, John Stephen, 1965-eng
dc.date.issued2005eng
dc.date.submitted2005 Falleng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file (viewed on October 18, 2007)eng
dc.descriptionVita.eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionThesis (Ph. D.) University of Missouri-Columbia 2005.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Physics.eng
dc.description.abstractAn atomic force microscope (AFM) was designed and built to study the mechanical properties of small collagen fibrils and the plasma membrane of living cells. Collagen is a major component of bone, skin and connective tissues, and is abundant in the extracellular matrix (ECM). Because of its abundance, an understanding of how disease affects collagen mechanics is crucial in disease prevention efforts. Two levels of type I collagen structure were investigated, subfibrils (on the order of 1 [mu] m in length) and longer fibrils. Comparisons were made between measurements of wild-type (wt) collagen and collagen from the mouse model of osteogenesis imperfecta (OI). Significant differences between OI and wt collagen were observed, primarily that intermolecular bonds in OI collagen fibrils are weaker than in wt, or not ruptured, as in the case of OI subfibrils. As cells interact with collagen in the ECM, the mechanical properties of the plasma membrane are also of great interest. Membrane tethers were extracted from living cells under varied conditions in order to assess the contributions of membrane-associated macromolecules such as the actin cytoskeleton and the glycocalyx, as well as intracellular signaling processes triggered by thapsigargin. Tether extraction force was found to be sensitive to all of these altered conditions, suggesting that tether extraction may be used to monitor various cellular processes.eng
dc.identifier.merlin.b60595565eng
dc.identifier.oclc174966906eng
dc.identifier.otherGrahamJ-121205-D3743eng
dc.identifier.urihttp://hdl.handle.net/10355/4191eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations.eng
dc.subject.lcshAtomic force microscopyeng
dc.subject.lcshOsteogenesis imperfectaeng
dc.subject.lcshCell membraneseng
dc.subject.lcshExtracellular matrix proteinseng
dc.subject.lcshCollageeng
dc.titleMechanical properties of complex biological systems using AFM-based force spectroscopyeng
dc.typeThesiseng
thesis.degree.disciplinePhysics (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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