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dc.contributor.authorGraham, John Stephen, 1965-en
dc.date.issued2005eng
dc.date.submitted2005 Fallen
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.en_US
dc.descriptionTitle from title screen of research.pdf file (viewed on October 18, 2007)en_US
dc.descriptionVita.en_US
dc.descriptionIncludes bibliographical references.en_US
dc.descriptionThesis (Ph. D.) University of Missouri-Columbia 2005.en_US
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Physics.en_US
dc.description.abstractAn atomic force microscope (AFM) was designed and built to study the mechanical properties of small collagen fibrils and the plasma membrane of living cells. Collagen is a major component of bone, skin and connective tissues, and is abundant in the extracellular matrix (ECM). Because of its abundance, an understanding of how disease affects collagen mechanics is crucial in disease prevention efforts. Two levels of type I collagen structure were investigated, subfibrils (on the order of 1 [mu] m in length) and longer fibrils. Comparisons were made between measurements of wild-type (wt) collagen and collagen from the mouse model of osteogenesis imperfecta (OI). Significant differences between OI and wt collagen were observed, primarily that intermolecular bonds in OI collagen fibrils are weaker than in wt, or not ruptured, as in the case of OI subfibrils. As cells interact with collagen in the ECM, the mechanical properties of the plasma membrane are also of great interest. Membrane tethers were extracted from living cells under varied conditions in order to assess the contributions of membrane-associated macromolecules such as the actin cytoskeleton and the glycocalyx, as well as intracellular signaling processes triggered by thapsigargin. Tether extraction force was found to be sensitive to all of these altered conditions, suggesting that tether extraction may be used to monitor various cellular processes.en_US
dc.identifier.merlin.b60595565en_US
dc.identifier.oclc174966906en_US
dc.identifier.otherGrahamJ-121205-D3743en_US
dc.identifier.urihttp://hdl.handle.net/10355/4191
dc.publisherUniversity of Missouri--Columbiaen_US
dc.relation.ispartof2005 Freely available dissertations (MU)en_US
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2005 Dissertations
dc.subject.lcshAtomic force microscopyen_US
dc.subject.lcshOsteogenesis imperfectaen_US
dc.subject.lcshCell membranesen_US
dc.subject.lcshExtracellular matrix proteinsen_US
dc.subject.lcshCollageen_US
dc.titleMechanical properties of complex biological systems using AFM-based force spectroscopyen_US
dc.typeThesisen_US
thesis.degree.disciplinePhysicsen_US
thesis.degree.disciplinePhysicseng
thesis.degree.grantorUniversity of Missouri--Columbiaen_US
thesis.degree.levelDoctoralen_US
thesis.degree.namePh. D.en_US


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