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dc.contributor.advisorLaughlin, M. Haroldeng
dc.contributor.advisorHamilton, Marceng
dc.contributor.authorVan Vickle, Gregory D., 1978-eng
dc.date.issued2005eng
dc.date.submitted2005 Summereng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionVita.eng
dc.description"August 2005"eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionThesis (M.S.) University of Missouri-Columbia 2005.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Veterinary biomedical sciences.eng
dc.description.abstractThe purpose of this thesis was to determine if specific components of oxidized low density lipoprotein (OxLDL) molecules e.g. oxidized polyunsaturated fatty acids (PUFAs), or saturated free fatty acids (FFA) are alone sufficient to decrease endothelial cells. This hypothesis was tested by incubating porcine aortic endothelial cells (PAECs) with various oxidized PUFAs and saturated FFAs. Twenty hour exposure of PAECs with the oxidized PUFAs (0-100[mu]M) linoleic acid, eicosapentaenoic acid, and arachidonic acid, does not alter eNOS protein content. However, PAEC incubation with the saturated FFAs (0-.6mM) palmitic and stearic acid, which do not undergo oxidative modification, dose dependently decrease eNOS content. The results of this study indicate that oxidized PUFAs are not sufficient alone to contribute to NO mediated endothelial dysfunction induced by OxLDL. However, saturated FFA may contribute to NO mediated endothelial dysfunction by decreased eNOS protein content within endothelial cells.eng
dc.identifier.merlinb55147434eng
dc.identifier.urihttp://hdl.handle.net/10355/4238
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.subject.lcshVascular endotheliumeng
dc.subject.lcshStem cellseng
dc.subject.lcshFatty acidseng
dc.subject.lcshVeterinary medicineeng
dc.titleThe effect of free fatty acids on endothelial cellseng
dc.typeThesiseng
thesis.degree.disciplineVeterinary biomedical sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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