Shared more. Cited more. Safe forever.
    • advanced search
    • submit works
    • about
    • help
    • contact us
    • login
    View Item 
    •   MOspace Home
    • University of Missouri-Columbia
    • Graduate School - MU Theses and Dissertations (MU)
    • Theses and Dissertations (MU)
    • Theses (MU)
    • 2005 Theses (MU)
    • 2005 MU theses - Freely available online
    • View Item
    •   MOspace Home
    • University of Missouri-Columbia
    • Graduate School - MU Theses and Dissertations (MU)
    • Theses and Dissertations (MU)
    • Theses (MU)
    • 2005 Theses (MU)
    • 2005 MU theses - Freely available online
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    advanced searchsubmit worksabouthelpcontact us

    Browse

    All of MOspaceCommunities & CollectionsDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis SemesterThis CollectionDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis Semester

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular AuthorsStatistics by Referrer

    Role of P2Y₂ nucleotide receptors in reactive astrogliosis

    Wang, Min
    View/Open
    [PDF] public.pdf (67.57Kb)
    [PDF] short.pdf (57.73Kb)
    [PDF] research.pdf (1.351Mb)
    Date
    2005
    Format
    Thesis
    Metadata
    [+] Show full item record
    Abstract
    Astrocytes become activated in response to brain injury characterized by increased expression of GFAP and increased rates of cell migration and proliferation. Damage to brain cells causes the release of cytoplasmic nucleotides, such as ATP and UTP, ligands for P2 nucleotide receptors. Results in this study with primary rat astrocytes indicate that activation of a G protein-coupled P2Y2 receptor for ATP and UTP increases GFAP expression and both chemotactic and chemokinetic cell migration. UTP-induced astrocyte migration was inhibited by silencing of P2Y2R expression with P2Y2R siRNA. UTP also increased the expression in astrocytes of [alpha]V[beta]3/5 integrins that are known to interact directly with the P2Y2R to modulate its function. Anti-[alpha]V integrin antibodies prevented UTP-stimulated astrocyte migration, suggesting that P2Y2R/[alpha]V interaction mediates the activation of astrocytes by UTP. P2Y2R-mediated astrocyte migration required the activation of the PI3-K/Akt and MEK/ERK signaling pathways, responses that also were inhibited by anti-[alpha]V integrin antibody. These results suggest that P2Y2Rs and their associated signaling pathways may be important factors regulating astrogliosis in brain disorders.
    URI
    http://hdl.handle.net/10355/4268
    Degree
    M.S.
    Thesis Department
    Neuroscience (MU)
    Collections
    • 2005 MU theses - Freely available online
    • Interdisciplinary Neuroscience Program electronic theses and dissertations (MU)

    Send Feedback
    hosted by University of Missouri Library Systems
     

     


    Send Feedback
    hosted by University of Missouri Library Systems