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dc.contributor.advisorWeisman, Gary A.eng
dc.contributor.authorWang, Mineng
dc.date.issued2005eng
dc.date.submitted2005 Springeng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file viewed on (January 22, 2007)eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionThesis (M.S.) University of Missouri-Columbia 2005.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Neuroscience.eng
dc.description.abstractAstrocytes become activated in response to brain injury characterized by increased expression of GFAP and increased rates of cell migration and proliferation. Damage to brain cells causes the release of cytoplasmic nucleotides, such as ATP and UTP, ligands for P2 nucleotide receptors. Results in this study with primary rat astrocytes indicate that activation of a G protein-coupled P2Y2 receptor for ATP and UTP increases GFAP expression and both chemotactic and chemokinetic cell migration. UTP-induced astrocyte migration was inhibited by silencing of P2Y2R expression with P2Y2R siRNA. UTP also increased the expression in astrocytes of [alpha]V[beta]3/5 integrins that are known to interact directly with the P2Y2R to modulate its function. Anti-[alpha]V integrin antibodies prevented UTP-stimulated astrocyte migration, suggesting that P2Y2R/[alpha]V interaction mediates the activation of astrocytes by UTP. P2Y2R-mediated astrocyte migration required the activation of the PI3-K/Akt and MEK/ERK signaling pathways, responses that also were inhibited by anti-[alpha]V integrin antibody. These results suggest that P2Y2Rs and their associated signaling pathways may be important factors regulating astrogliosis in brain disorders.eng
dc.identifier.merlinb57669557eng
dc.identifier.urihttp://hdl.handle.net/10355/4268
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartof2005 Freely available theses (MU)eng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.subject.lcshAstrocyteseng
dc.subject.lcshBrain -- Wounds and injurieseng
dc.subject.lcshNucleotideseng
dc.subject.lcshCell migrationeng
dc.titleRole of P2Y₂ nucleotide receptors in reactive astrogliosiseng
dc.typeThesiseng
thesis.degree.disciplineNeuroscience (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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