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dc.contributor.advisorMitchum, Melissa G.eng
dc.contributor.authorYeckel, Gregory Josepheng
dc.contributor.otherUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Theses. 2012 Theseseng
dc.date.issued2012eng
dc.date.submitted2012 Falleng
dc.descriptionTitle from PDF of title page; abstract from short PDF (University of Missouri--Columbia, viewed on June 26, 2014).eng
dc.description.abstractPlants resistant to the soybean cyst nematode (SCN) mount a hypersensitive cell death-like response upon nematode feeding, but the genes regulating this process are not known. Laser-assisted microdissection of nematode feeding cells coupled with microarray analysis identified a soybean gene upregulated 87-fold in plants resistant to SCN that shared sequence similarity with the Arabidopsis BAG6 (Bcl-2 associated athanogene 6) gene. BAG genes encode an evolutionarily conserved family of proteins in animals, yeast and plants. These proteins contain a conserved BAG domain which mediates interaction with the molecular chaperone HSP70/HSC70. Members of the BAG protein family in animals and yeast function in apoptosis to regulate a range of activities from inhibition to promotion of cell death. However, much less is known about the role of BAG proteins in plants. A family of seven BAG genes (AtBAG1-7) has been identified in Arabidopsis. AtBAG6 was shown to induce programmed cell death in both yeast and Arabidopsis. Expression of a truncated version of the protein, spanning a calmodulin-binding IQ domain and the BAG domain, enhanced the cell death phenotype. Here, we demonstrate that similar to AtBAG6, overexpression of the full length GmBAG6A protein or a truncated version spanning the IQ and BAG domains, induced cell death in yeast and plants, with the truncated form showing an enhanced cell death phenotype. Expression of the truncated form in Arabidopsis and soybean under the control of a nematode-inducible promoter significantly reduced nematode development demonstrating its potential use in engineering a novel form of nematode resistance in crop plants.eng
dc.format.extentpageseng
dc.identifier.urihttp://hdl.handle.net/10355/43355eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartof2012 Freely available theses (MU)eng
dc.titleCharacterization of a soybean BAG gene and its potential role in nematode resistanceeng
dc.typeThesiseng
thesis.degree.disciplinePlant, insect and microbial sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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