A novel PCR based DNA microanalyzer system for detection of viral genome

MOspace/Manakin Repository

Breadcrumbs Navigation

A novel PCR based DNA microanalyzer system for detection of viral genome

Please use this identifier to cite or link to this item: http://hdl.handle.net/10355/4336

[-] show simple item record

dc.contributor.advisor Gangopadhyay, Shubhra en
dc.contributor.advisor Grant, Sheila Ann en
dc.date.accessioned 2010-01-12T17:05:16Z
dc.date.available 2010-01-12T17:05:16Z
dc.date.issued 2006 en_US
dc.identifier.other BhattacharyaS-071806-D5349 en_US
dc.identifier.uri http://hdl.handle.net/10355/4336
dc.description The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. en_US
dc.description Title from title screen of research.pdf file viewed on (April 25, 2007) en_US
dc.description Includes bibliographical references. en_US
dc.description Vita. en_US
dc.description Thesis (Ph.D.) University of Missouri-Columbia 2006. en_US
dc.description Dissertations, Academic -- University of Missouri--Columbia -- Biological engineering. en_US
dc.description.abstract A micro-fluidic assay to quickly analyze microscopic samples of DNA is being developed for field applications. It consists of a micro-PCR chamber, micropumps, and micro-heaters. Additional components of the device include gel electrophoresis microchannels and solid core waveguide fluorescence collectors. The intended analyzer is a micro-fluidic platform that is principally based on the three-step polymerase chain reaction (PCR) mechanism. Currently, all off chip control is executed using a labview code. The micro-pumps, PCR chamber and capillary electrophoresis system have been designed fabricated and tested. For fabrication of the device, a regime has been developed for bonding PDMS surfaces to a variety of substrates (silicon in the present case). We have successfully achieved a compression in the cycle time by a factor of ten in our on chip PCR reactor as compared to the conventional PCR system and also amplify samples with pico-gram concentration. Fluorescent studies indicate negligible non-specific binding to our chip which has been a major problem in earlier assays. A working electrophoretic capillary and new biphasic gel material with extremely low background and high signal to noise ratio have been developed. We have further achieved low voltage capillary electrophoresis by doping different gel materials with conducting nano-particles. We envision this assay as a highly sensitive field deployable analyzer tool. en_US
dc.language.iso en_US en_US
dc.publisher University of Missouri--Columbia en_US
dc.subject.lcsh Polymerase chain reaction en_US
dc.subject.lcsh DNA -- Analysis en_US
dc.subject.lcsh Microchemistry en_US
dc.title A novel PCR based DNA microanalyzer system for detection of viral genome en_US
dc.type Thesis en_US
thesis.degree.discipline Biological engineering en_US
thesis.degree.grantor University of Missouri--Columbia en_US
thesis.degree.name Ph.D. en_US
thesis.degree.level Doctoral en_US
dc.identifier.merlin .b5845892x en_US
dc.identifier.oclc 123497011 en_US
dc.relation.ispartofcommunity University of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2006 Dissertations
dc.relation.ispartofcollection 2006 Freely available dissertations (MU)


This item appears in the following Collection(s)

[-] show simple item record