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dc.contributor.advisorGangopadhyay, Shubhraen
dc.contributor.advisorGrant, Sheila Annen
dc.date.issued2006eng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.en_US
dc.descriptionTitle from title screen of research.pdf file viewed on (April 25, 2007)en_US
dc.descriptionIncludes bibliographical references.en_US
dc.descriptionVita.en_US
dc.descriptionThesis (Ph.D.) University of Missouri-Columbia 2006.en_US
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biological engineering.en_US
dc.description.abstractA micro-fluidic assay to quickly analyze microscopic samples of DNA is being developed for field applications. It consists of a micro-PCR chamber, micropumps, and micro-heaters. Additional components of the device include gel electrophoresis microchannels and solid core waveguide fluorescence collectors. The intended analyzer is a micro-fluidic platform that is principally based on the three-step polymerase chain reaction (PCR) mechanism. Currently, all off chip control is executed using a labview code. The micro-pumps, PCR chamber and capillary electrophoresis system have been designed fabricated and tested. For fabrication of the device, a regime has been developed for bonding PDMS surfaces to a variety of substrates (silicon in the present case). We have successfully achieved a compression in the cycle time by a factor of ten in our on chip PCR reactor as compared to the conventional PCR system and also amplify samples with pico-gram concentration. Fluorescent studies indicate negligible non-specific binding to our chip which has been a major problem in earlier assays. A working electrophoretic capillary and new biphasic gel material with extremely low background and high signal to noise ratio have been developed. We have further achieved low voltage capillary electrophoresis by doping different gel materials with conducting nano-particles. We envision this assay as a highly sensitive field deployable analyzer tool.en_US
dc.identifier.merlin.b5845892xen_US
dc.identifier.oclc123497011en_US
dc.identifier.otherBhattacharyaS-071806-D5349en_US
dc.identifier.urihttp://hdl.handle.net/10355/4336
dc.publisherUniversity of Missouri--Columbiaen_US
dc.relation.ispartofcollection2006 Freely available dissertations (MU)
dc.relation.ispartofcommunityUniversity of Missouri-Columbia. Graduate School. Theses and Dissertations. Dissertations. 2006 Dissertations
dc.source.originalSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subject.lcshPolymerase chain reactionen_US
dc.subject.lcshDNA -- Analysisen_US
dc.subject.lcshMicrochemistryen_US
dc.titleA novel PCR based DNA microanalyzer system for detection of viral genomeen_US
dc.typeThesisen_US
thesis.degree.disciplineBiological engineering (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh.D.eng


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