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dc.contributor.advisorRucker, Edmund B. IIIeng
dc.contributor.advisorPrather, Randall S.eng
dc.contributor.authorIsom, Stephen Clay, 1975-eng
dc.date.issued2006eng
dc.date.submitted2006 Springeng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file viewed on (February 27, 2007)eng
dc.descriptionVita.eng
dc.descriptionThesis (Ph.D.) University of Missouri-Columbia 2006.eng
dc.description.abstractIt was the objective of these experiments to more fully characterize the biochemical and developmental responses of in vitro-produced preimplantation stage porcine embryos to elevated temperatures. In vitro fertilized (IVF) and parthenogenetically activated (PA) embryos were exposed to elevated temperatures (42 C) for nine hours during the late one-cell stage of development. The IVF embryos demonstrated significant susceptibility to the applied heat stress, both in their developmental status (20.6% and 8.8% of embryos developed to blastocysts in the heat stressed [HS] and non-heat stressed [NHS] groups respectively; P < 0.05) and their apoptotic response (26.7% of NHS embryos, and 45.6% of HS embryos with at least one apoptotic blastomere; P < 0.05). The PA embryos were resistant to the effects of elevated temperature as applied in these experiments (P > 0.1 for NHS versus HS embryos for development to blastocyst and apoptosis rates). When exposed to different timing variations of this same heat stress, PA embryos that were heat treated immediately after oocyte activation demonstrated a much accelerated first cell cycle (63% of HS embryos cleaved by 24 hours-post-activation [hpa], as compared to 23.5% of control embryos; P < 0.0001), and significantly higher blastocyst rates (38.0% and 18.5% for HS and NHS embryos, respectively, for example; P < 0.005) as compared to control embryos and embryos heat treated at later timepoints. The obvious developmental differences detected in PA embryos heat treated as described were not associated with any apparent aberrations in the timing of Maturation Promoting Factor (MPF) inactivation. Mitogen-Activated Protein Kinase (MAPK) inactivation, however, occurred much earlier in heat stressed embryos (between 3 and 6 hpa) than in control embryos (between 6 and 9 hpa). Artificial maintenance of high levels of MAPK activity served to abolish the developmental discrepancies between HS and NHS embryos. Thus, the enhanced developmental potential of HS PA embryos appears to be correlated to precocious inactivation of MAPK. A more complete understanding of this phenomenon may lead to techniques that can ultimately improve the efficiency with which porcine embryos are produced in vitro.eng
dc.description.bibrefIncludes bibliographical referenceseng
dc.identifier.merlinb57900334eng
dc.identifier.oclc85373216eng
dc.identifier.urihttps://doi.org/10.32469/10355/4385eng
dc.identifier.urihttps://hdl.handle.net/10355/4385
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsOpenAccess.eng
dc.subject.lcshSwine -- Embryos -- Transplantationeng
dc.subject.lcshTemperature -- Physiological effecteng
dc.titleThe effects of elevated temperature on preimplantation-stage porcine embryoseng
dc.typeThesiseng
thesis.degree.disciplineAnimal sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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