[-] Show simple item record

dc.contributor.advisorPhillips, Charlotte L.eng
dc.contributor.authorPfeiffer, Brent J., 1975-eng
dc.date.issued2006eng
dc.date.submitted2006 Springeng
dc.descriptionTitle from title screen of research.pdf file (viewed on December 22, 2006).eng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionVita.eng
dc.description"May 2006"eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionThesis (Ph. D.) University of Missouri-Columbia 2006.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biochemistry (Medicine).eng
dc.description.abstractThe extracellular matrix (ECM) is an important constituent for a variety of tissues including vascular tissue in which the ECM maintains aortic wall integrity. An important component of vascular tissue ECM is type I collagen. Type I collagen is normally a molecule composed of three collagen chains of which two are the same chain [proa1(I)] and one is distinctly different [proa2(I)]. The focus of this dissertation is to examine the role of the proa2(I) chain in determining thoracic aorta integrity and how the thoracic aortic integrity changes with age. To assess the role of proa2(I) chains we used a mouse model, termed 'oim', that produces only proa1(I) chains and evaluated thoracic aortas of our mouse model at 3, 8, and 18 months old of age. We evaluated thoracic aortic strength, stiffness, ECM content, ECM gene expression, and collagen crosslinking at each age point. Oim mice exhibited reduced aortic strength and stiffness at each age group and exhibited increased aortic strength and stiffness at 18 months of age compared to 3 months of age. Oim mice also exhibited reduced aortic collagen content, while other aortic ECM components were unchanged. However, aortic collagen content was significantly increased at 8 and 18 months of age as compared to 3 months of age. Aortic ECM gene expression demonstrated reduced expression at 18 months of age as compared to 3 months of age. In addition, oim aortas demonstrated increased collagen crosslinks at each age group, while the ratio of collagen crosslinking remained the same at each age group. Our results demonstrate that proa2(I) collagen is central for proper aortic strength and stiffness even in the presence of increased collagen crosslinking and increasing collagen content of homotrimeric type I collagen with age. This study suggests that fibrils composed of homotrimeric type I collagen are inherently weaker than fibrils composed of heterotrimeric type I collagen.eng
dc.identifier.merlin.b57475179eng
dc.identifier.oclc77524168eng
dc.identifier.otherPfeifferB-042006-D5064eng
dc.identifier.urihttp://hdl.handle.net/10355/4397eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.sourceSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subject.lcshCollagen -- Metabolismeng
dc.subject.lcshBlood -- Circulationeng
dc.subject.lcshAorta -- Mechanical propertieseng
dc.subject.meshCollagen Type I -- metabolismeng
dc.subject.meshAorta, Thoracic -- metabolismeng
dc.subject.meshAorta, Thoracic -- physiopathologyeng
dc.subject.meshCollagen Type I -- deficiencyeng
dc.subject.meshMice, Mutant Strainseng
dc.subject.meshOsteogenesis Imperfecta -- geneticseng
dc.subject.meshOsteogenesis Imperfecta -- metabolismeng
dc.titleRole of Proa(2)I collagen chains and collagen crosslinking in thoracic aortic biochemical integrity during aging using the OIM mouse modeleng
dc.typeThesiseng
thesis.degree.disciplineBiochemistry (Medicine) (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


Files in this item

[PDF]
[PDF]
[PDF]

This item appears in the following Collection(s)

[-] Show simple item record