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dc.contributor.advisorTaylor, Kristen H.eng
dc.contributor.authorBurmeister, Daneeng
dc.date.issued2013eng
dc.date.submitted2013 Summereng
dc.descriptionJuly 2013.eng
dc.descriptionA Thesis presented to the Faculty of the Graduate School at the University of Missouri--Columbia In Partial Fulfillment of the Requirements for the Degree Master of Science.eng
dc.descriptionThesis supervisor: Dr. Kristen H. Taylor.eng
dc.descriptionIncludes vita.eng
dc.description.abstractHeritable changes to DNA conformation can have significant impacts on gene expression without altering the DNA sequence. The RUN domain containing 3B gene (RUNDC3B) has been shown to be differentially expressed in cancer types such as, acute lymphoblastic leukemia. This differential expression has been linked to methylation of the gene. Combined bisulfite restriction analysis and methylation specific PCR were used to determine the methylation status of five regions spanning a CpG island (CGI) in the promoter region of RUNDC3B.This analysis was completed in cell lines from lymphoid malignancies, myeloid malignancies, various solid tumor cancers, and healthy lymphoid cell lines. To determine the effect of aberrant DNA methylation on RUNDC3B expression, real-time PCR was utilized. The lymphoid malignancies were found to have more prominent methylation and express RUNDC3B at lower levels than other malignant and healthy lymphoid cell lines. Sequence analysis of the CGI in the RUNDC3B promoter (RUNDC3B CGI) was performed to support the importance of this region as a regulatory element in several species. In this project we have extended our understanding of aberrant RUNDC3B CGI methylation to include cell lines representing 15 malignancies. Our data suggest that methylation located in the RUNDC3B CGI may serve as a biomarker for lymphoid malignancies. Additionally, the results provide the basis for continued analysis of the role of RUNDC3B repression in the pathogenesis of lymphoid malignancies.eng
dc.description.bibrefIncludes bibliographical references (pages 50-53).eng
dc.format.extent1 online resource (viii, 68 pages) : illustrations (some color)eng
dc.identifier.oclc898039394eng
dc.identifier.urihttps://hdl.handle.net/10355/43989
dc.identifier.urihttps://doi.org/10.32469/10355/43989eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.sourceSubmitted by the University of Missouri--Columbia Graduate Schooleng
dc.subject.lcshLymphomaseng
dc.subject.lcshDNA -- Methylationeng
dc.subject.lcshEpigeneticseng
dc.subject.lcshLeukemiaeng
dc.subject.lcshLymphoid tissue -- Cancereng
dc.titleAberrant DNA methylationof RUN domain containing 3B in lymphoid malignancieseng
dc.title.alternativeAberrant DNA methylation of RUN domain containing 3B in lymphoid malignancieseng
dc.typeThesiseng
thesis.degree.disciplinePathology (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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