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dc.contributor.advisorRogers, Elizabeth E.eng
dc.contributor.authorDurrett, Timothy P., 1976-eng
dc.date.issued2006eng
dc.date.submitted2006 Summereng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file (viewed on April 27, 2009)eng
dc.descriptionVita.eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionThesis (Ph.D.) University of Missouri-Columbia 2006.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biochemistry (Agriculture)eng
dc.description.abstractTwo projects studying different aspects of iron deficiency in the model plant Arabidopsis thaliana are detailed here. The first project describes the isolation and characterization of the Arabidopsis frd4-1 and frd4-2 mutants that do not induce Fe(III) chelate reductase activity in their roots in response to iron deficiency. Map-based cloning revealed that the frd4 mutations reside in cpFtsY, which encodes a component of one of the pathways responsible for the insertion of proteins into the thylakoid membranes of the chloroplast. A number of different hypotheses were tested in an attempt to explain how defects in cpFtsY could affect the expression of root Fe(III) chelate reductase activity. The second project involves the further characterization of the protein FRD3, which was previously shown to be important for the efficient translocation of iron from roots to the shoots. Xylem exudate from frd3 plants contains significantly less citrate and iron than the exudate from wild type plants. Additionally, supplementation of growth media with citrate rescues the frd3 phenotypes. The ectopic expression of FRD3-GFP results in enhanced exudation of citrate from roots. Finally, heterologous studies in Xenopus laevis oocytes reveal that FRD3 mediates the transport of citrate. These results strongly support the hypotheses that FRD3 effluxes citrate into the root vasculature, a process important for the translocation of iron to the leaves, and that iron moves through the xylem as a ferric-citrate complex.eng
dc.identifier.merlinb67108647eng
dc.identifier.oclc319430172eng
dc.identifier.urihttps://hdl.handle.net/10355/4437
dc.identifier.urihttps://doi.org/10.32469/10355/4437eng
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.sourceSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subjectfrd3; frd4-1; frd4-2.eng
dc.subjectfrd3; frd4-1; frd4-2eng
dc.subject.lcshIron deficiency diseases in plantseng
dc.subject.lcshArabidopsis thaliana -- Effect of iron oneng
dc.subject.lcshHomeostasiseng
dc.titleUnderstanding Arabidopsis ion homeostasis in the post-genomic era: assigning function to two proteins involved in iron metabolismeng
dc.typeThesiseng
thesis.degree.disciplineBiochemistry (Agriculture) (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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