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dc.contributor.advisorPhillips, Charlotte L.eng
dc.contributor.authorCarleton, Stephanie M., 1979-eng
dc.date.issued2006eng
dc.date.submitted2006 Falleng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file (viewed on May 1, 2009)eng
dc.descriptionVita.eng
dc.descriptionThesis (Ph.D.) University of Missouri-Columbia 2006.eng
dc.description.abstractOsteogenesis imperfecta (OI) is a diverse disease of type I collagen, the main structural protein in the body. It has been well documented that related individuals harboring the same OI-causing mutation can have very different clinical outcomes. However, the relationship between genotype and clinical outcome remains unclear. The goal of this study was to further examine the genotype-phenotype relationship with the long-term goal of identifying modifier genes that impact the quality of bone. Modifier genes influence clinical outcome by interacting with genes known to impact bone quality such as the type I collagen genes. As gene expression changes throughout development, we sought to determine if age-related changes in bone quality occurred. To this end, the same OI-causing mutation was studied on two genetic backgrounds, one with reduced bone strength (C57) and one with variable bone strength (outbred). Wildtype and oim animals of each strain were then analyzed at one, two and four months of age to assess bone shape and strength as well as biochemical parameters. This work showed strain related differences at all ages examined, but the presence of the oim mutation overrides these differences. Bones from oim animals of either strain have altered bone geometry and reduced biomechanical strength as compared to wildtype, with C57-oim animals having a more severe phenotype than outbred oim animals. Oim animals also had altered mineral composition and reduced amounts of type I collagen, which were strain specific. Taken together, these data indicate a role for genetic background in determining phenotypic severity, although some parameters are more affected by genetic background than others.eng
dc.description.bibrefIncludes bibliographical references.eng
dc.identifier.merlinb67208265eng
dc.identifier.oclc319861617eng
dc.identifier.oclc319861617eng
dc.identifier.urihttps://doi.org/10.32469/10355/4474eng
dc.identifier.urihttps://hdl.handle.net/10355/4474
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcommunityUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.rightsOpenAccess.eng
dc.subject.lcshOsteogenesis imperfectaeng
dc.subject.lcshGeneticseng
dc.subject.lcshPhenotypeeng
dc.titleThe role of genetic background on the phenotypic severity of the osteogensis imperfecta murine (oim) COLIA2 gene mutation throughout postnatal developmenteng
dc.typeThesiseng
thesis.degree.disciplineGenetic area program (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelDoctoraleng
thesis.degree.namePh. D.eng


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