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dc.contributor.advisorSegal, Steven S.eng
dc.contributor.authorCanine, Curteng
dc.date.issued2010eng
dc.date.submitted2010 Summereng
dc.description"December 2010."eng
dc.description"A Thesis presented to the Faculty of the Graduate School at the University of Missouri--Columbia In Partial Fulfillment of the Requirements for the Degree Master of Science."eng
dc.descriptionThesis supervisor: Dr. Steven Segal.eng
dc.description.abstractSkeletal muscle arterioles dilate in response to application of acetylcholine (ACh), eliciting a conducted vasodilation (CVD) that travels along unbranched segments without decrement. CVD is known to entail cell-to-cell transmission of hyperpolarization along the endothelium via gap junction channels, a purely passive mechanism. In the present thesis I study CVD in bifurcating arteriolar networks, where the pathway for hyperpolarizing current expands compared to unbranched arterioles, to test for an active component to CVD. In a separate subset of arterioles, the effect of augmenting vasomotor tone on CVD was tested using elevated O2 or phenylephrine (PE) in the superfusion solution vs. control. Male C57BL/6 mice (n=13; 10-13 weeks old) were anesthetized with pentobarbital sodium (50mg/kg, intraperitoneal injection) and maintained at 37 [degrees]C. The cremaster was carefully exteriorized and spread onto a transparent Sylgard pedestal. The tissue was maintained at 34 [degrees]C with continuous superfusion of physiologic saline solution. Microiontophoresis of ACh evoked non-decremental CVD in both unbranched and bifurcating arterioles, supporting the role of an active component in CVD. Further, augmenting vasomotor tone with PE attenuated non-decremental CVD, whereas increasing vasomotor tone with elevated O2 did not alter CVD. In summary, arteriolar networks in the mouse cremaster muscle exhibit robust dilation to ACh, which conducts along arterioles and across branch points without decrement, suggesting the contribution of both active and passive mechanisms. In preparations exhibiting poor spontaneous vasomotor tone, elevated O2 can be used to improve resting tone without impacting CVD.eng
dc.description.bibrefIncludes bibliographical references (pages 51-58).eng
dc.format.extent1 online resource (ix, 58 pages) : illustrationseng
dc.identifier.oclc900559520eng
dc.identifier.urihttps://hdl.handle.net/10355/44847
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.rightsOpenAccess.eng
dc.rights.licenseThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
dc.subjectPhysiology (Medicine)eng
dc.titleThe control of blood flow in skeletal muscle arterioleseng
dc.typeThesiseng
thesis.degree.disciplineMedical Pharmacology and Physiology (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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