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dc.contributor.advisorPrather, Randall S.eng
dc.contributor.authorMartin, Lisa M.eng
dc.date.issued2006eng
dc.date.submitted2006 Springeng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file viewed on (February 9, 2007)eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionThesis (M.S.) University of Missouri-Columbia 2006.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Animal sciences..eng
dc.description.abstractNuclear transfer (NT) is an inefficient but invaluable tool. This study examines abnormalities associated with NT porcine embryos at days 10, 12, and 14. Methods: 4 experimental groups were examined: non-pregnant, in vivo pregnant, NT recipients, and manipulation control (MC) recipients. Maternal blood samples were collected and assayed for insulin-like growth factor-1 (IGF-1). Embryos were evaluated for embryonic disc diameter, morphology, nucleoli density and mitotic figure index. Results: NT day 12 (P < 0.03) and day 14 (P < 0.01) embryos had increased nucleoli when compared to in vivo produced and MC embryos. NT day 14 embryos had an increased (P < 0.03) mitotic index when compared to in vivo produced day 14 embryos. In vivo produced day 12 embryos had greater (P < 0.03) embryonic disk diameters compared to NT and MC embryos; however in vivo produced day 14 embryos had increased (P < 0.01) embryonic disk diameters only when compared to NT day 14 embryos. In vivo produced day 14 embryos were morphologically more advanced (P < 0.01) than NT and MC day 14 embryos. There were no significant differences between IGF-1 levels. Conclusions: Nuclear transfer embryos develop at a slower rate than in vivo embryos with abnormalities potentially affecting the cell cycle. The techniques used during nuclear transfer also appear to compromise embryo development.eng
dc.identifier.merlinb57719433eng
dc.identifier.urihttp://hdl.handle.net/10355/4623
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.subject.lcshCell nuclei -- Transplantationeng
dc.subject.lcshSwine -- Embryoseng
dc.titleMorphologic and histologic comparisons between in vivo and nuclear transfer derived porcine embryoseng
dc.typeThesiseng
thesis.degree.disciplineAnimal sciences (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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