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dc.contributor.advisorSeye, Cheikh I.eng
dc.contributor.advisorWeisman, Gary A.eng
dc.contributor.authorJain, Nishant Rajkumareng
dc.date.issued2006eng
dc.date.submitted2006 Summereng
dc.descriptionThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file.eng
dc.descriptionTitle from title screen of research.pdf file (viewed on April 21, 2009)eng
dc.descriptionIncludes bibliographical references.eng
dc.descriptionThesis (M.S.) University of Missouri-Columbia 2006.eng
dc.descriptionDissertations, Academic -- University of Missouri--Columbia -- Biochemistry (Agriculture)eng
dc.description.abstractExtracellular nucleotides can bind to the P2Y₂R and modulate proliferation and migration of smooth muscle cells, which is known to be involved in intimal hyperplasia that accompanies atherosclerosis and post-angioplasty restenosis. Moreover, the P2Y₂R is upregulated in vascular smooth muscle cells and endothelial cells in response to tissue injury. These findings suggest that the P2Y₂R is a potential target for the pharmacological control of progression of atherosclerosis and post-angioplasty restenosis. However, the mechanisms governing P2Y₂R up-regulation remain unknown. In this study, we have cloned a 2071 bp 5'-flanking region of the P2Y₂R gene in a reporter vector and carried out a serial deletion analysis. The deletion of a 175 bp region completely abolished promoter function and results further indicate that the P2Y₂R gene promoter uses an array of positive and negative response elements in the regulation of gene expression. Furthermore, other results show that the cytokine IL-1[beta] may be involved in down-regulation of P2Y₂R activity in human coronary artery endothelial cells. Further studies will potentially lead to the identification of novel pathways involved in the regulation of P2Y₂R gene expression, information that might be useful to suppress neointimal hyperplasia in atherosclerosis and the restenosis of angioplasty.eng
dc.identifier.merlinb66822257eng
dc.identifier.urihttp://hdl.handle.net/10355/4629
dc.languageEnglisheng
dc.publisherUniversity of Missouri--Columbiaeng
dc.relation.ispartofcollectionUniversity of Missouri--Columbia. Graduate School. Theses and Dissertationseng
dc.source.originalSubmitted by University of Missouri--Columbia Graduate School.eng
dc.subject.lcshNucleotideseng
dc.subject.lcshAdenosine triphosphateeng
dc.subject.lcshPurines -- Receptorseng
dc.subject.lcshG proteinseng
dc.subject.lcshGene expressioneng
dc.titleCharacterization and functional analysis of the P2Y₂R gene promotereng
dc.typeThesiseng
dc.type.genreElectronic bookseng
dc.type.genreElectronic dissertationseng
dc.type.genreFreely available online resourceseng
thesis.degree.disciplineBiochemistry (Agriculture) (MU)eng
thesis.degree.grantorUniversity of Missouri--Columbiaeng
thesis.degree.levelMasterseng
thesis.degree.nameM.S.eng


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