Shared more. Cited more. Safe forever.
    • advanced search
    • submit works
    • about
    • help
    • contact us
    • login
    View Item 
    •   MOspace Home
    • University of Missouri-Columbia
    • Graduate School - MU Theses and Dissertations (MU)
    • Theses and Dissertations (MU)
    • Dissertations (MU)
    • 2007 Dissertations (MU)
    • 2007 MU dissertations - Freely available online
    • View Item
    •   MOspace Home
    • University of Missouri-Columbia
    • Graduate School - MU Theses and Dissertations (MU)
    • Theses and Dissertations (MU)
    • Dissertations (MU)
    • 2007 Dissertations (MU)
    • 2007 MU dissertations - Freely available online
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.
    advanced searchsubmit worksabouthelpcontact us

    Browse

    All of MOspaceCommunities & CollectionsDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis SemesterThis CollectionDate IssuedAuthor/ContributorTitleIdentifierThesis DepartmentThesis AdvisorThesis Semester

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular AuthorsStatistics by Referrer

    Optical glucose nanobiosensor encapsulated in erythrocytes

    El-Dweik, Majed, 1966-
    View/Open
    [PDF] public.pdf (2.159Kb)
    [PDF] short.pdf (11.79Kb)
    [PDF] research.pdf (2.491Mb)
    Date
    2007
    Format
    Thesis
    Metadata
    [+] Show full item record
    Abstract
    An implantable glucose biosensor encapsulated in erythrocytes, Red Blood Cells (RBC), will become a method for continuously measuring blood glucose concentration in diabetics. In 2005, the American Diabetes Association (ADA) reported that 20.8 million people have diabetes, making it the fifth leading cause of death by disease in the USA. This paper focuses on the preparation phase of the glucose sensor. Glucose Binding Protein (GBP) from E. coli was labeled with two fluorophores, Alexa Fluor 680 (AF680), and Alexa Fluor 750 (AF750). This sensor based on Fluorescence Resonance Energy Transfer (FRET). FRET is a distance sensitive technique between the above fluorophores. The initial energy transfer between AF680 and AF750 labeled on the GBP before glucose additions was determined. After glucose additions, the labeled GBP went through conformational change which caused distance between the labeled sites. This change in distance caused a change in the energy transfer. The labeled GBP became the glucose nanobiosensor. The labeled GBP nanobiosensors were encapsulated in erythrocytes, red blood cells (RBCs), by using the Hypo-Osmotic dialysis technique. The encapsulated RBCs responded well to different glucose concentrations ranging form 0-33.16mM. This range covers the normal blood glucose concentration, 4 - 9mM.
    URI
    https://doi.org/10.32469/10355/4672
    https://hdl.handle.net/10355/4672
    Degree
    Ph. D.
    Thesis Department
    Biological engineering (MU)
    Rights
    OpenAccess.
    This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
    Collections
    • Biological Engineering electronic theses and dissertations - CAFNR (MU)
    • 2007 MU dissertations - Freely available online
    • Biological Engineering electronic theses and dissertations - Engineering (MU)

    Send Feedback
    hosted by University of Missouri Library Systems
     

     


    Send Feedback
    hosted by University of Missouri Library Systems