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    • University of Missouri-Columbia
    • Graduate School - MU Theses and Dissertations (MU)
    • Theses and Dissertations (MU)
    • Dissertations (MU)
    • 2007 Dissertations (MU)
    • 2007 MU dissertations - Freely available online
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    Determination of total selenium and seleno-amino acids in yeast and aquatic organisms by liquid chromatography and inductively coupled plasma mass spectrometry

    Wan, Lili, 1976-
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    [PDF] research.pdf (557.1Kb)
    Date
    2007
    Format
    Thesis
    Metadata
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    Abstract
    The primary goal of this dissertation research was to develop methods for routine speciation analysis of selenium in tissues. A method for total selenium using on-line stable isotope dilution analysis with conventional ICP-MS (SIDA-ICP-MS) was developed. Masses at 77, 78, 79, 81, and 82 were monitored and quantitation of Se was determined based on both 78Se and 82Se. SIDA-ICP-MS was successfully applied to the determination of total selenium in biological materials, such as selenized yeasts, certified reference materials, lab-cultured oligochaetes and desert pupfish. The separation and quantitation of seleno-amino acids was accomplished by ion-pairing reversed-phase liquid chromatography (RPLC) and detected by ICP-MS at mass 82 using the standard mode. It was found that methanesulfonic acidic hydrolysis demonstrated a higher extraction efficiency of selenomethionine than enzymatic digestion. Selenomethionine (SeMet) was the only significant Se-containing species detected in the biological samples that were examined.
    URI
    https://hdl.handle.net/10355/4683
    https://doi.org/10.32469/10355/4683
    Degree
    Ph. D.
    Thesis Department
    Chemistry (MU)
    Rights
    OpenAccess.
    This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License.
    Collections
    • Chemistry electronic theses and dissertations (MU)
    • 2007 MU dissertations - Freely available online

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